Full Name: Thrombosis Profile ELISA Kit
Sample Type: Serum, Plasma
Sensitivity: 1.0 U/ml
Thrombosis refers to the formation of a blood clot or thrombus within the circulatory system. Clot formation is triggered when the endothelium is damaged, exposing the subendothelial layer. This activates platelets which aggregate at the site of injury and provide a surface for coagulation factors to assemble. Tissue factor interacts with factor VIIa to initiate the extrinsic pathway, while exposed collagen activates the intrinsic pathway. This generates thrombin which converts fibrinogen into fibrin strands that stabilize the clot.
Platelet activation is amplified by locally released factors like ADP, thromboxane A2, and thrombin. Platelets change shape, release granules, and express integrins and receptors. The major platelet receptors are GPVI, GPIb-IX-V, and αIIbβ3 integrin. Activated platelets adhere to exposed matrix proteins via these receptors.
Anticoagulant and fibrinolytic systems counterbalance clot formation. Healthy endothelium prevents thrombosis through molecules like thrombomodulin, heparan sulfate, nitric oxide, and prostacyclin. Dysfunction of anticoagulant mechanisms promotes thrombosis. Causative factors include hypercoagulability, endothelial injury, stasis of blood flow, and abnormal platelet function.
Human thrombosis profile ELISA kit is designed for detecting in-vitro quantitative levels of IgG or IgM autoantibodies to phospholipids and beta-2 glycoprotein I (thrombosis profile) in human plasma and serum. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.
Highly purified human beta-2 glycoprotein I, cardiolipin, phosphatidyl inositol, phosphatidyl serine and phosphatidic acid are bound to individual rows of the microwell plate.
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Divisible Microplate: Consisting of 12 modules of 8 wells, highly purified trombosis antibodies bound to microwells.
- Calibrators (A-F): Concentration 0-100 U/ml, made up from anti-phospholipids in serum/buffer matrix.
- Sample Buffer (5x).
- Enzyme Conjugates: Contains anti-human IgG and anti-human IgM antibodies, HRP labelled.
- TMB Substrate.
- Stop Solution: Contains acid.
- Wash Buffer (50x Concentrated).
- Instruction Manual.
- Certificate of Analysis.
The minimum detection sensitivity level of human beta-2 glycoprotein I and phospholipids IgG/IgM autoantibodies (thrombosis profile) using current thrombosis profile IgG/IgM ELISA kit was 1.0 U/ml. The dynamic range for this assay is 6.3 – 100.0 U/ml.
– Results Interpretation: IgG and IgM (Normal < 10 U/ml, Elevated ≥ 10 U/ml).
– Specificity: Highly specific for b2-Glycoprotein I, phospholipid and the complex of the negatively charged phospholipid.
– Calibration: Against internationally recognised reference sera.
– Parallelism: Displays linearity over the complete measuring range.
– Interference: None.
- Management of recurrent thrombosis in antiphospholipid syndrome. Curr Rheumatol Rep. (2014) 16 (3): 405. Review. Nalli C., et al.
- Antiphospholipid antibody profile: implications for the evaluation and management of patients. Lupus. (2010) 19 (4): 432-5. Tincani A., et al.
- Prevention of thrombosis in antiphospholipid syndrome. Hematology Am Soc Hematol Educ Program. (2016) 2016 (1): 707-713. Review. Lim W.
- Primary thrombosis prophylaxis in antiphospholipid antibody-positive patients: where do we stand? Curr Rheumatol Rep. (2011) 13 (1): 59-69. Review. Barbhaiya M. and Erkan D.
- Certoparin for the treatment and prevention of thrombosis: pharmacological profile and results from clinical studies. Expert Opin Drug Metab Toxicol. (2013) 9 (7): 901-9. Review. Donadini M.P., et al.
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