Phosphatidyl Serine Ab ELISA Kit

Full Name: Phosphatidyl Serine Ab ELISA Kit
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: IgM APS: 2.8 u/ml, IgG APS: 1.0 u/ml

BACKGROUND

Phosphatidyl sеrinе is a phospholipid which is a kеy componеnt of thе cеll mеmbranе, it plays an important function in cеll cyclе signalling that is spеcifically rеlatеd to apoptosis. Phospholipids that usually surround our cеlls tеnd to bе vital molеculеs which arе providing cеllular structurе and protеction. Many publications havе also indicatеd that it is critical for hormonе sеcrеtion by thе adrеnal glands, bonе matrix formation, hеartbеat coordination, tеsticular function, cеll rеpair and rеmoval by thе immunе systеm. Many publications havе found that phosphatidyl-sеrinе can improvеd cognitivе bеhaviours and abilitiеs, howеvеr, this bеnеfit only lastеd for a fеw months. Somе of thе hеalth bеnеfits of phosphatidylsеrinе includе improving symptoms of Alzhеmеimеr’s disеasе, combats dеprеssion, improvеs Parkinson’s disеasе symptoms and aids thе slowdown of agе-rеlatеd cognitivе dеclinе.

INTENDED USE

Human phosphatidyl serine antibody ELISA kit is designed for detecting in-vitro quantitative amounts IgG and IgM auto-antibodies to anti-phosphatidylserine IgG/IgM (phosphatidylserine antibody, phosphatidyl serine Ab) in human plasma and serum. This assay has a minimum analytical sensitivity limit of IgG APS: 1.0 u/ml, IgM APS: 2.8 u/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiterstrips (12 x 8 Wells): Coated with phosphatidyl serine purified from bovine spinal cord.
• Enzyme Conjugate: Anti-IgG and Anti-IgM, conjugated with horseradish peroxidase.
• Calibrators: IgG: 60 u/ml, IgM: 60 u/ml.
• Control Sera (Positive): For IgG and IgM, dilute 1:100.
• Control Serum (Negative): Dilute 1:100.
• Wash Buffer (20x Dilute Concentrate).
• Sample Diluent.
• TMB Substrate.
• Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of phosphatidylserine IgG/IgM (phosphatidyl serine antibody, phosphatidylserine Ab) auto-antibodies using current human phosphatidyl serine ELISA kit was IgG APS: 1.0 u/ml, IgM APS: 2.8 u/ml.

ASSAY CHARACTERISTICS

– Results Interpretation: Negative (IgG < 25 u/ml, IgM < 14 u/ml), Low Positive (IgG 25 – 35 u/ml, IgM 14 – 20 u/ml), Moderate Positive (IgG 36 – 50 u/ml, IgM 21 – 25 u/ml), High (IgG > 51 u/ml, IgM > 26 u/ml).
– Intra Assay: IgG 4.9 – 7.8%, IgM 3.3 – 11.1%
– Inter Assay: IgG 2.5 – 4.7%, IgM 3.9 – 9.5%

REFERENCES

1. Antibodies to phosphatidylserine/prothrombin complex and the antiphospholipid syndrome. Lupus. (2014) 23 (12): 1309-12. Sciascia S. and Bertolaccini M.L.
2. “New” antigenic targets and methodological approaches for refining laboratory diagnosis of antiphospholipid syndrome. J Immunol Res. (2015) 2015: 858542. Review. Misasi R., et al.
3. Antiphospholipid antibodies: discovery, definitions, detection and disease. Prog Lipid Res. (2003) 42 (3): 176-237. Review. McIntyre J.A., et al.
4. Antibodies to Phosphatidylserine/Prothrombin Complex in Antiphospholipid Syndrome: Analytical and Clinical Perspectives. Adv Clin Chem. (2016) 73: 1-28. Review. Peterson L.K., et al.

OTHER RELATED ELISA KITS

• Phospholipid Screen ELISA Kit
• Insulin ELISA Kit
• Prothrombin IgG/IgM ELISA Kit