Tetracycline ELISA Kit

Full Name: Tetracycline ELISA Kit
Sample Type: Food (Cheese, Meat, Milk, Shrimps, Honey, Milk Powder)
Sensitivity: 0.024 ng/ml

BACKGROUND

The Actinobacteria genus Streptomyces produces the polyketide antibiotic tetracycline. It inhibits bacterial growth by binding reversibly to the bacterial 30S ribosomal subunit and preventing incoming aminoacyl tRNA from binding to the ribosome acceptor site. It also binds to some extent to the bacterial 50S ribosomal subunit and may cause intracellular components to leak from bacterial cells by altering the cytoplasmic membrane.

Tetracycline is an antibiotics that is effectively used in the treatment of infections of the respiratory tract, intestines, urinary tract and chlamydia. However, the general usefulness for this chemical has become less popular due to the widespread development of antibiotic resistance in causative organisms. Despite this, tetracycline is a popular choice of treatment for many specific indications, this includes for example brucellosis, syphilis, malaria, cholera, plague and acne.

In many countries tetracycline is also accepted in food production and therefore consuming tetracycline could lead to potential risks. This has resulted in most countries regulating the maximum amount of tetracycline residues present in foods.

INTENDED USE

Tetracycline ELISA kit is a procedure designed for rapid quantitative detection of tetracycline in present in food (such as cheese, milk, milk powder, honey, shrimps and meat). This assay has a minimum analytical sensitivity limit of 0.024 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate: Coated with anti tetracycline antibody.
• Tetracycline Standards 1-6 (100x): Concentration 0, 0.04, 0.1, 0.4, 1, 4 ng/ml.
• Conjugate (Tetracycline-Peroxidase).
• Substrate Solution (TMB).
• Stop Solution (0.5 M H2SO4).
• Extraction and sample dilution buffer (Tris)(10x).
• Washing Solution (PBS + Tween 20)(10x Concentrate).
• Instruction Manual.

SENSITIVITY

The minimum detection sensitivity level of tetracycline using current tetracycline ELISA kit was 0.024 ng/ml. The standard range for this assay is 0.04 – 4.0 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity: Limit of detection, LOD (0.024ng/ml), Limit of quantification, LOQ (0.072ng/ml)
– Recovery: Honey (103%), Milk (94%), Non-fat Dry Milk (90%), Shrimps (89%), Meat (87%), Cheese (82%).
– Linearity: 83 – 110%
– Intra-Assay Precision: 4%
– Inter-Assay Precision: 13%
– Reactivity: 4-Epitetracycline (111%), Tetracycline (100%), Rolitetracycline (82%), Chlortetracycline (42%), Doxycycline (41%), Demeclocycline (37%), Oxytetracycline (34%), 4-Epioxytetracycline (34%), 4-Epichlortetracycline (11%), Methacycline (9%), Minocycline (1%).

REFERENCES

1. Application of ELISA in determining the fate of tetracyclines in land-applied livestock wastes. Analyst. (2003) 128 (6): 658-62. Aga D.S., et al.
2. Quantitative detection of tetracycline-residues in honey by a simple sensitive immunoassay. Anal Chim Acta. (2008) 626 (2): 180-5. Jeon M. and Rhee Paeng I.
3. A direct competitive assay-based aptasensor for sensitive determination of tetracycline residue in honey. Talanta. (2015) 131: 562-9. Wang S., et al.
4. Preparation of antibodies and development of an enzyme-linked immunosorbent assay (ELISA) for the determination of doxycycline antibiotic in milk samples. J Agric Food Chem. (2012) 60 (15): 3837-46. Adrian J., et al.

OTHER RELATED ELISA KITS

• Chloramphenicol ELISA Kit
• Penicillin ELISA Kit
• Streptomycin ELISA Kit