Tetracycline ELISA kit is a procedure designed for rapid quantitative detection of tetracycline in present in food (such as cheese, milk, milk powder, honey, shrimps and meat). This assay has a minimum analytical sensitivity limit of 0.024 ng/ml.
Tetracycline belong to a wide range of antibiotics which are often used in treating infections of the respiratory tract, intestines and urinary tract, they are also useful in the treatment of chlamydia in particular those patients that have an allergy to macrolides and β-lactams. However, the general usefulness for this chemical has become less popular due to the widespread development of antibiotic resistance in causative organisms. Despite this, tetracycline is a popular choice of treatment for many specific indications, this includes for example brucellosis, syphilis, malaria, cholera, plague and acne.
Tetracycline works by blocking the ability of bacteria to produce proteins, through binding specific prokaryotic ribosomes. In many countries tetracycline is also accepted in food production and therefore consuming tetracycline could lead to potential risks. This has resulted in most countries regulating the maximum amount of tetracycline residues present in foods.
TETRACYCLINE ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Plate: Coated with anti tetracycline antibody.
- Tetracycline Standards 1-6 (100x): Concentration 0, 0.04, 0.1, 0.4, 1, 4 ng/ml.
- Conjugate (Tetracycline Peroxidase).
- Substrate Solution (TMB).
- Stop Solution (0.5 M H2SO4).
- Extraction and sample dilution buffer (Tris)(10x).
- Washing Solution (PBS + Tween 20)(10x Concentrate).
- Instruction Manual.
The minimum detection sensitivity level of tetracycline using current tetracycline ELISA kit was 0.024 ng/ml. The standard range for this assay is 0.04 – 4.0 ng/ml.
– Tetracycline: ELISA
– Sensitivity: Limit of detection, LOD (0.024ng/ml), Limit of quantification, LOQ (0.072ng/ml)
– Recovery: Honey (103%), Milk (94%), Non-fat Dry Milk (90%), Shrimps (89%), Meat (87%), Cheese (82%).
– Linearity: 83 – 110%
– Intra-Assay Precision: 4%
– Inter-Assay Precision: 13%
– Reactivity: 4-Epitetracycline (111%), Tetracycline (100%), Rolitetracycline (82%), Chlortetracycline (42%), Doxycycline (41%), Demeclocycline (37%), Oxytetracycline (34%), 4-Epioxytetracycline (34%), 4-Epichlortetracycline (11%), Methacycline (9%), Minocycline (1%).
- Application of ELISA in determining the fate of tetracyclines in land-applied livestock wastes. Analyst. (2003) 128 (6): 658-62. Aga D.S., et al.
- Quantitative detection of tetracycline residues in honey by a simple sensitive immunoassay. Anal Chim Acta. (2008) 626 (2): 180-5. Jeon M. and Rhee Paeng I.
- A direct competitive assay-based aptasensor for sensitive determination of tetracycline residue in honey. Talanta. (2015) 131: 562-9. Wang S., et al.
- Preparation of antibodies and development of an enzyme-linked immunosorbent assay (ELISA) for the determination of doxycycline antibiotic in milk samples. J Agric Food Chem. (2012) 60 (15): 3837-46. Adrian J., et al.
- Non-antibiotic properties of tetracyclines as anti-allergy and asthma drugs. Pharmacol Res. (2011) 64 (6): 602-9. Review. Joks R. and Durkin H.G.
- Full Name: Tetracycline ELISA Kit
- Sample Type: Food (Cheese, Meat, Milk, Shrimps, Honey, Milk Powder)
- Sensitivity: 0.024 ng/ml