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Chloramphenicol ELISA Kit

Full Name: Chloramphenicol ELISA Kit
Sample Type: Food, Urine
Sensitivity: 0.03 ng/ml

BACKGROUND

Chloramphenicol contains outstanding antibacterial properties and is therefore frequently used in the production of meat, milk and eggs. It can help in the detection of signs, symptoms and in some cases even offer therapy options. For example for eye ointment in order to treat conjunctivitis, oral or injected into a vein to treat plague, meningitis, typhoid fever and cholera. Due to its adverse effects it has been found to cause in humans which often can result in infection, inflammation, hypersensitivity and food allergy. Despite its adverse effects, chloramphenicol is inevitable in the treatment of many infections like Salmonella typhi. This is especially true for those who have genetically modified animals without any additional antibiotics which leaves them vulnerable to bacteria.

The use of chloramphenicol as an antibiotic or a vitamin is restricted and in a many situations it is prohibited especially during food safety and analytic studies. The following assay methodology offer’s many advantages over the conventional methods in that no radioactive material is required, the procedure is shorter and provides sensitivity that is better than chromatographic and comparable to radioimmunoassay. Two of the main methods which have been used for analysis are gas chromatography and radioimmunoassay.

INTENDED USE

Chloramphenicol ELISA kit is intended for measuring quantitative amounts of chloramphenicol present in food and urine samples. This assay has a minimum analytical sensitivity limit of 0.03 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Coated with an antibody binding protein.
  • Chloramphenicol Standards 1-6: Concentration 0, 0.05, 0.1, 0.5, 1.0, 5 ng/ml.
  • Anti-Chloramphenicol Antibody (sheep).
  • Conjugate (Chloramphenicol-Peroxidase).
  • Substrate Solution (TMB).
  • Stop Solution.
  • Sample Diluent (PBS).
  • Washing Solution (PBS + Tween 20)(10x).
  • Instruction Manual.

SENSITIVITY

The minimum detection sensitivity level of chloramphenicol using current chloramphenicol ELISA kit was 0.03 ng/ml. The standard range for this assay is 0.05 – 50.0 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity: 0.03 ng/ml
– Recovery: Meat (108%), Honey (98%), Milk (ethyl acetate extraction) (98%), Shrimps (96%), Whole Egg (95%), Milk (direct assay) (94%), Fish meal (90%), Urine (90%).
– Intra-Assay Variation: 8%
– Cross Reactivity: Relative to chloramphenicol (=100%): Chloramphenicol Glucuronide (88%), Chloramphenicol Base, Ampicillin, Penicillin, Tetracycline (< 0.1%).

REFERENCES

  1. Comparsion of an immunochromatographic strip with ELISA for simultaneous detection of thiamphenicol, florfenicol and chloramphenicol in food samples. Biomed Chromatogr. (2015) 29 (9): 1432-9. Guo L., et al.
  2. An ultrasensitive chemiluminescence immunoassay of chloramphenicol based on gold nanoparticles and magnetic beads. Drug Test Anal. (2013) 5 (5): 346-52. Tao X., et al.
  3. Characterisation of antibodies to chloramphenicol, produced in different species by enzyme-linked immunosorbent assayand biosensor technologies. Anal Chim Acta. (2007) 592 (1): 51-7. Fodey T., et al.
  4. Evaluation of matrix effect in isotope dilution mass spectrometry based on quantitative analysis of chloramphenicol-residues in milk powder. Anal Chim Acta. (2014) 807: 75-83. Li X.Q., et al.

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