Streptomycin ELISA kit is a procedure designed for determining quantitative amounts of streptomycin in food. This assay has a minimum analytical sensitivity limit of 1.0 ng/ml.
Streptomycin is an essential antibiotic which is often used to treat many bacterial infections (for example: tuberculosis, brucellosis, mycobacterium avium complex, tularemia, burkholderia infection, endocarditis and plague). Streptomycin is known to be naturally produced from the actinobacterium Streptomyces griseus and it is also activity is directed against tubercle bacillus and gram-negative bacteria. A number of common side effects include fever, rash, vomiting, heading spinning and numbness of the face. Streptomycin is also used in combination with penicillin as a standard antibiotic cocktail in order to prevent bacterial infection in cell cultures.
Due to its many side effects, streptomycin it is rarely used for human treatment, however, it has a vital application in the veterinary area. For example, following the treatment of mastitis in breeding animals, this was found to cause elevated streptomycin in milk, muscle, liver and kidney. Another relatively new application for antibiotic streptomycin is described as Plantomycin (treatment of fruit tree which are known as fire blight).
STREPTOMYCIN ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter plate: Coated with anti mouse antibody.
- Streptomycin Standards 1-6: Concentration 0, 2, 5, 20, 50, 200 ng/ml.
- Anti Streptomycin Antibody (mouse).
- Conjugate (Streptomycin-Peroxidase).
- Substrate Solution (TMB).
- Stop Solution (1 N acidic solution).
- Sample Diluent (PBS).
- Washing Solution (PBS + Tween 20)(10x Concentrate).
- Instruction Manual.
The minimum detection sensitivity level of streptomycin using current streptomycin ELISA kit was 1.0 ng/ml. The standard range for this assay is 2.0 – 200.0 ng/ml.
– Streptomycin: ELISA
– Sensitivity: 1.0 ng/ml (based on standard curve)
– Intra-Assay Precision: 6%
– Specificity (Cross Reactivity): Relative to streptomycin (=100%): Dihydrostreptomycin (70%), Gentamycin, Neomycin (< 0.001%).
– Recovery: Milk (120%), Honey (100%), Liver (95%), Meat (90%), Whole egg (85%), Shrimps (70%).
- Pretreatment-free immunochromatographic assay for the detection of streptomycin and its application to the control of milk and dairy products. Anal Chim Acta. (2011) 701 (2): 209-17. Byzova N.A., et al.
- Effect of streptomycin treatment on the shedding of and the serologic responses to Leptospira interrogans serovar hardjo subtype hardjobovis in experimentally infected cows. Vet Microbiol. (1993) 38 (1-2): 129-35. Gerritsen M.J., et al.
- Flow-injection determination of streptomycin residues in milk using the luminol-periodate-Mn2+ chemiluminescence system. Luminescence. (2006) 21 (1): 36-42. Wan G.H., et al.
- Determination of streptomycin residues in food by solid-phase extraction and liquid chromatography with post-column derivatization and fluorometric detection. J Chromatogr A. (1999) 830 (2): 345-51. Edder P., et al.
- One-tube loop-mediated isothermal amplification combined with restriction endonuclease digestion and ELISA for colorimetric detection of resistance to isoniazid, ethambutol and streptomycinin Mycobacterium tuberculosis isolates. J Microbiol Methods. (2010) 83 (1): 53-8. Lee M.F., et al.
- Full Name: Streptomycin ELISA Kit
- Sample Type: Food
- Sensitivity: 1.0 ng/ml