Streptomycin ELISA Kit

Full Name: Streptomycin ELISA Kit
Sample Type: Food
Sensitivity: 1.0 ng/ml

BACKGROUND

Streptomycin is used to treat many bacterial infections for example; tuberculosis, brucellosis, mycobacterium avium complex, tularemia, burkholderia infection, endocarditis and plague. This antibiotic is used in combination therapy. It may be given by injection into a vein or muscle, or orally. Inhaled use is also possible. Inhaled treatment may be done using nebulized formulations, which are more effective than dry powder preparation. The dose for children ranges from 10 to 20 mg/kg a day for several days to weeks. The usual adult dose is 100-125 mg/day divided twice daily, as 50-100 mg every six hours or 250-500 mg every four hours.

Streptomycin is known to be naturally produced from the actinobacterium Streptomyces griseus and it is also activity is directed against tubercle bacillus and gram-negative bacteria. A number of common side effects include fever, rash, vomiting, heading spinning and numbness of the face. Due to its many side effects, it is rarely used for human treatment, however, it has a vital application in the veterinary area. For example, following the treatment of mastitis in breeding animals, this was found to cause elevated streptomycin in milk, muscle, liver and kidney. Another relatively new application for antibiotic streptomycin is described as Plantomycin (treatment of fruit tree which are known as fire blight).

INTENDED USE

Streptomycin ELISA kit is a procedure designed for determining quantitative amounts of streptomycin in food. This assay has a minimum analytical sensitivity limit of 1.0 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter plate: Coated with anti mouse antibody.
• Streptomycin Standards 1-6: Concentration 0, 2, 5, 20, 50, 200 ng/ml.
• Anti Streptomycin Antibody (mouse).
• Conjugate (Streptomycin-Peroxidase).
• Substrate Solution (TMB).
• Stop Solution (1 N acidic solution).
• Sample Diluent (PBS).
• Washing Solution (PBS + Tween 20)(10x Concentrate).
• Instruction Manual.

SENSITIVITY

The minimum detection sensitivity level of streptomycin using current streptomycin ELISA kit was 1.0 ng/ml. The standard range for this assay is 2.0 – 200.0 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity: 1.0 ng/ml (based on standard curve)
– Intra-Assay Precision: 6%
– Specificity (Cross Reactivity): Relative to streptomycin (=100%): Dihydrostreptomycin (70%), Gentamycin, Neomycin (< 0.001%).
– Recovery: Milk (120%), Honey (100%), Liver (95%), Meat (90%), Whole egg (85%), Shrimps (70%).

REFERENCES

1. Pretreatment-free immunochromatographic assay for the detection of streptomycin and its application to the control of milk and dairy products. Anal Chim Acta. (2011) 701 (2): 209-17. Byzova N.A., et al.
2. Effect of streptomycin treatment on the shedding of and the serologic responses to Leptospira interrogans serovar hardjo subtype hardjobovis in experimentally infected cows. Vet Microbiol. (1993) 38 (1-2): 129-35. Gerritsen M.J., et al.
3. Flow-injection determination of streptomycin-residues in milk using the luminol-periodate-Mn2+ chemiluminescence system. Luminescence. (2006) 21 (1): 36-42. Wan G.H., et al.
4. Determination of streptomycin residues in food by solid-phase extraction and liquid chromatography with post-column derivatization and fluorometric detection. J Chromatogr A. (1999) 830 (2): 345-51. Edder P., et al.
5. One-tube loop-mediated isothermal amplification combined with restriction endonuclease digestion and ELISA for colorimetric detection of resistance to isoniazid, ethambutol and streptomycinin Mycobacterium tuberculosis isolates. J Microbiol Methods. (2010) 83 (1): 53-8. Lee M.F., et al.

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• Penicillin ELISA Kit
• Tetracycline ELISA Kit
• Chloramphenicol ELISA Kit