Equine PMSG ELISA Kit (Pregnant Mare Serum Gonadotrophin)

Full Name: Equine PMSG ELISA Kit (Pregnant Mare Serum Gonadotrophin)
Reactivity: Equine
Sample Type: Serum, Plasma
Range: 25.0 – 800.0 mIU/ml

BACKGROUND

PMSG or equine chorionic gonadotrophin is a hormone that is used alongside progesterone to induce ovulation in livestock before carrying out artificial insemination. Pregnant mares are found to secrete this hormone between the 40th and the 120th days of gestation, it is secreted by their endometrial cups and can be collected so that it is used to artificially induce estrus in the female cattle, sheep, swine and goats. The advantages are that it has a longer circulatory half-life, even though it is less pure than pituitary extracts. This offers a specific test for pregnancy because this hormone can only be present in pregnant mare. PMSG is a mixture of conjugated and unconjugated forms of progesterone and other hormones. It cannot be simply separated into the two parts because they are attached to each other by various side chains which gives it its characteristic structure. The unconjugated form is almost entirely testosterone, while the conjugates in PMSG are mainly estrogen, but also includes cortisol. This hormone can be extracted from urine using salicylic acid as a reagent.

PMSG can also be used to determine whether a female experienced an abortion or is pregnant. The urine of pregnant mares is higher in this hormone than non-pregnant females. In order to test for pregnancy, blood samples are taken from the ‘smear’ obtained by scraping a finger nail (a pre-packaged sample of blood) and then diluting it with sterile water then testing for the presence of PMSG. In horses, the PMSG gene is on chromosome 5. The PMSG gene is identical to the one in humans.

INTENDED USE

Equine PMSG ELISA kit is intended for quantitatively measuring specific pregnant mare serum gonadotrophin (PMSG) levels in equine (horse) plasma or serum. The dynamic range for this assay is 25.0 – 800.0 mIU/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Wells: Coated with anti-PMSG antibody (monoclonal).
• Standard Zero (0).
• Standard (Standard 1-5).
• Enzyme Conjugate.
• TMB Substrate Solution.
• Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of pregnant mare serum using current equine PMSG ELISA kit as per instruction manual. The dynamic range which can be expected using this assay is 25.0 – 800.0 mIU/ml.

ASSAY CHARACTERISTICS

– Intra Assay: 3.2 – 8.2%
– Inter Assay: 7.3 – 9.8%
– Recovery: 93 – 112%
– Linearity: 91 – 105%
– Interfering Substances: Cross reactivity with LH and FSH is eliminated using serum or plasma samples at a high dilution (1:100) with zero standard.
– High-Dose-Hook Effect: No hook effect was observed in this test.

REFERENCES

1. Immunochemical study of equine chorionic gonadotropin (eCG/PMSG): antigenic determinants on alpha- and beta-subunits. Biochim Biophys Acta. (1992) 1159 (1): 74-80. Maurel M.C., et al.
2. Radio-immunoassay for PMSG and its application to in-vivo studies. J Reprod Fertil. (1979) 55 (2): 339-45. Menzer C. and Schams D.
3. Influence of PMSG and time of artificial insemination on fertility of progestogen-treated sheep in confinement. J Anim Sci. (1982) 54 (6): 1205-11. Langford G.A.
4. Laboratory methods of equine pregnancy diagnosis. Vet Rec. (1977) 100 (19): 396-9. Review. Walker D.

OTHER RELATED ELISA KITS

• PMSG Rapid Test
• Progesterone ELISA Kit (Rat/Mouse)
• Prolactin ELISA Kit (Rat)