Feline Total T4 ELISA Kit (Thyroxine)

Full Name: Feline Total T4 ELISA Kit (Thyroxine)
Reactivity: Feline
Sample Type: Plasma, Serum
Range: 50.0 – 250.0 nmol/l


Thyroxine (T4) problems in cats can be identified through the following symptoms; polyphagia, polyuria, polydipsia, weight loss, cardiac disease, vomiting and hyperactivity. Usually, all of these symptoms point to hyperthyroidism. Surgery to remove the thyroid gland or thyroid gland ligation to reduce thyroid hormone levels can be used to treat hyperthyroid cats. The signs and symptoms of T4 problems can include behaviour changes such as nervousness, excessive vocalization and/or restlessness, weight loss with or without appetite changes, lethargy and uneven coat texture.

Thyroxine problems can occur in cats due to autoimmune thyroiditis (the body attacking the thyroid gland) or cancerous growths on the gland. Furthermore, they may not create enough of their own hormones to maintain a normal level or the hormones that are produced may not be entirely functioning. It is recommended that T4 blood tests are performed for both hyperthyroidism with antibodies and hypothyroidism before surgery is done.

Cats are found to not lose their appetite and are also quite active even though they are displaying the above symptoms. In majority of cases (approx. 80%) there is an enlargement of one or even both thyroid lobes that can be found. There are 2 types of thyroid tumors benign or malignant. Benign tumors are usually small in size and may not produce symptoms beyond weight loss or an enlargement, whereas, malignant tumors can grow quite large and cause complications such as; compression of nearby organs and pain in the neck especially if they involve the carotid artery which is just below the collarbone. Treatment involves either surgery to remove the tumor or medical management with medication that can help regulate hormone levels.


Feline total T4 ELISA kit is designed to detect quantitative levels of total thyroxine (T4)  using feline serum or plasma samples. The dynamic range for this assay is 50.0 – 250.0 nmol/l.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Strips: Coated monoclonal anti-T4 total antibodies.
  • Strip Holder.
  • ELISA Buffer.
  • Conjugate Buffer.
  • Standard (1-4): Concentration 0, 50, 100, 250 nmol/L.
  • Wash Solution (200X concentrated).
  • Substrate A.
  • Substrate B.
  • Stop Solution.
  • Plastic Cover Seal.


The standard range of total thyroxine (T4) using current feline total T4 ELISA kit is 50.0 – 250.0 nmol/l. Please contact us for the protocol insert for more details.


  1. Measurement of total thyroxine concentration in serum from dogs and cats by use of various methods. Am J Vet Res. (2006) 67 (2): 259-65. Kemppainen R.J. and Birchfield J.R.
  2. Feline focus: Diagnostic testing for feline thyroid disease: hyperthyroidism. Compend Contin Educ Vet. (2013) 35 (8): E3. Peterson M.E.
  3. Effect of illness not associated with the thyroid gland on serum total and free thyroxine concentrations in cats. J Am Vet Med Assoc. (1996) 208 (12): 2004-8. Mooney C.T., et al.
  4. Combined assessment of serum free and total T4 in a general clinical setting seemingly has limited potential in improving diagnostic accuracy of thyroid dysfunction in dogs and cats. Vet Clin Pathol. (2014) 43 (1): 1-3. Rasmussen S.H., et al.


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