sVCAM-1 ELISA Kit (Soluble Vascular Cell Adhesion Molecule-1)

INTENDED USE

Human sVCAM-1 ELISA kit is a procedure for analysing in vitro quantitative concentrations of soluble vascular cell adhesion molecule-1 (sVCAM1, CD106, sVCAM-1) in human cell culture supernatant, serum, biological fluids, cell lysates and plasma. This assay has a minimum sensitivity limit of 0.05 ng/ml.

BACKGROUND

VCAM-1 (CD106 or vascular cell adhesion molecule-1) is a 90-kDa transmembrane glycoprotein. It is made of six or seven immunoglobulin domains. One of its main roles is to mediate the adhesion process of the immune cells to vascular endothelium cells during inflammation. It is a protein that is important for mediating adhesion of the following cells vascular endothelium, lymphocytes, eosinophils and basophils.

Soluble forms (sVCAM-1) are likely products that are derived from a number of different sources such; as enzymatic cleavage from tumor, endothelial and leukocyte cell surfaces. Elevated serum levels have been recently linked with disease progression in patients with; leukemia, colorectal, renal, gastric prostate, bladder and ovarian cancers.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with sVCAM-1.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of soluble vascular cell adhesion molecule-1 (sVCAM-1, CD106, sVCAM1) using current sVCAM-1 ELISA kit was 0.05 ng/ml. The dynamic range for this assay is 0.1 – 30 ng/ml.

REFERENCES

1. Soluble adhesion molecules in pediatric rheumatic diseases. J Rheumatol. (2002) 29 (4): 832-6. Bloom B.J., et al.
2. Circulating serum sVCAM-1 concentration in advanced ovarian cancer patients: correlation with concentration in ascites. Radiol Oncol. (2014) 48 (3): 307-13. Jakimovska M., et al.
3. Adhesion molecules and atherosclerosis. Atherosclerosis. (2003) 170 (2): 191-203. Review. Blankenberg S., et al.
4. Cell adhesion molecules: potential therapeutic diagnostic implications. Mol Biotechnol. (2008) 38 (1): 33-40. Review. Mousa S.A.

ADDITIONAL INFORMATION

• Full Name: sVCAM-1 ELISA Kit (Soluble Vascular Cell Adhesion Molecule-1)
• Reactivity: Human
• Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids
• Sensitivity: 0.05 ng/ml

OTHER RELATED ELISA KITS

• VCAM-1 ELISA Kit
• sPECAM-1 ELISA Kit
• sICAM-1 ELISA Kit