SAA ELISA Kit (Serum Amyloid A)

INTENDED USE

Human  SAA ELISA kit can be used for detecting in vitro quantitative concentrations of serum amyloid A (SAA) in human plasma, various biological fluids, tissue homogenates and serum samples. This assay has a minimum detection limit of 0.938 ng/ml.

BACKGROUND

Serum amyloid A (SAA) is a 104 amino acid polypeptide which in its native state has a molecular mass between 12-14 kDa. It is also a precursor of amyloid A (AA) protein (approx. 8.5 kDa) that can be produced following the cleavage of the first 76 amino acids. The gene is found to be located on chromosome 11p and the human protein can exist in a number of polymorphic forms (SAA1 to SAA4). It has been identified as an acute-phase protein, which during acute events results in rapid elevation of SAA levels, in addition to an intense increase in all the other acute phase proteins.

In general SAA circulates at trace levels (between levels of 1-5 µg/mL) under normal conditions; however following 4-6 hours after an inflammatory stimulus, this causes the levels to rapidly elevate to greater than 1000 fold (approx. 500-1000 µg/mL). This response makes it a very sensitive marker for many inflammatory stimulus. High levels are found in patients that experience acute and chronic inflammation.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-human SAA antibody.
• Standards: Concentrations 0, 1.562, 3.125, 6.25, 12.5, 25, 50, 100 ng/ml, made up from lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-Labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of serum amyloid A (SAA) using this SAA ELISA kit was 0.938 ng/ml. The dynamic range for this assay is 1.563 – 100 ng/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for SAA, no cross reactivity or interference between SAA and analogues was detected.
– Recovery: Serum (88 – 100%), EDTA Plasma (88 – 104%), Heparin Plasma (85 – 97%).
– Linearity: Serum (96 – 100%), EDTA Plasma (84 – 101%), Heparin Plasma (82 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Serum amyloid A (SAA): a concise review of biology, assay methods and clinical usefulness. Clin Chem Lab Med. (1999) 37 (4): 381-8. Review. Yamada T.
2. SAA, an apoprotein of HDL: its structure and function. Ann N Y Acad Sci. (1982) 389: 183-9. Review. Benditt E.P., et al.
3. The cytokine-serum amyloid A-chemokine network. Cytokine Growth Factor Rev. (2016) 30: 55-69. Review. De Buck M., et al.

ADDITIONAL INFORMATION

• Full Name: SAA ELISA Kit (Serum Amyloid A)
• Reactivity: Human
• Sample Type: Tissue Homogenates, Serum, Other Biological Fluids, Plasma
• Sensitivity: 0.938 ng/ml

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