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C-Reactive Protein (CRP) ELISA Kit

INTENDED USE

Human CRP ELISA kit is designed for measuring in vitro quantitative amounts of c-reactive protein (CRP) in human plasma and serum samples. This assay has a minimum detection limit of < 1.0 µg/ml.

CRP BACKGROUND

CRP is an acute phase response predominant protein, its blood levels have been used as an index of ongoing inflammatory responses including those that occur in cancer. CRP can also be elevated on injury that causes necrosis and tissue breakdowns and have been observed following major surgery such as myocardial infarction and cerebral infarction.

CRP testing is done mainly for the medical diagnosis, and to monitor and detect health conditions linked to inflammation. Physiologically, the role of CRP is to bind to phosphocholine that is on the surface of dying or dead cells (and also some forms of bacteria).

C-reactive protein (known as CRP for short) is an acute phase protein that was first discovered and named due to its ability to precipitate the c-polysaccharide of pneumococcus in calcium. It is essentially classified as a superfamily of proteins that are known as pentraxins or pentaxins and these contain cyclic, non-glycosylated structures which are made up of apparently five identical globular non-covalently linked subunits that can be aggregated symmetrically.

CRP elisa kits are a commonly and widely used application for the detection and quantification of proteins and antigens from samples taken. C reactive protein is a serum protein that is produced by the liver. CRP levels rise in a range of acute and inflammatory conditions and act as an acute inflammation marker. The inflammatory conditions also include infection, cardiovascular disease, tissue injury, malignancy, and inflammatory disease. CRP is present in the active stages of inflammatory disorders like ankylosing spondylitis, rheumatoid arthritis, Crohn’s disease, Reiter’s syndrome, systemic lupus erythematosus, polyarthritis, psoriatic arthropathy and ulcerative colitis.

When used in conjugation with myeloperoxidase, CRP monitoring and assessment is used clinically as an accurate predictor of mortality for patients with cardiac issues.

Each of these subunits are 206 amino acids (23.05 kD) and the total molecular weight of CRP is 117.5 kDa, consisting of 14 anti-parallel beta-strands that are arranged in two beta-sheets form.

During the acute phase response, the concentration of CRP can increase up to 1000-fold within a few hours. Whereas, normally in human biological fluids it is only present in trace amounts (approx. 0.6 mg/L). Certain cytokines (IL-1 alpha, IL-1 beta, TNF-alpha or beta and indirectly by IL-6) are responsible for stimulating CRP, which results in dramatically enhancing its synthesis by hepatocytes. It has a rapid response time, short half-life and large incremental change. Among the acute phase proteins, CRP is the most sensitive, fast-reacting and the easiest to measure, also its catabolism is not affected by the type of inflammation.

In addition, serum concentration levels of CRP are found to significantly increase for infectious or non-infectious inflammations, for necrosis or tissue damage and during the presence of many malignant tumours.

CRP ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Coated Microplate: Pre-coated with monoclonal antibodies to human CRP.
  • Standards (A-E): Concentrations 0, 525, 50, 100 µg/ml. Calibrated against the NIBSC 1st International Standard, 85/506.
  • Enzyme Conjugate.
  • Specimen/Sample Dilution Buffer.
  • Washing Solution (20x).
  • TMB Chromogen Solution.
  • Stopping Solution.

Contact us for price and shipping information and further product details. We also have some free shipping options that could be applied for large orders in the UK.

SENSITIVITY

The minimum detection sensitivity level of human c-reactive protein (CRP) using current CRP ELISA kit was 1.0 µg/ml. The dynamic range for this assay is 5.0 – 100.0 µg /ml. It is recommended to follow the user manual guide in order to achieve the expected results.

ASSAY CHARACTERISTICS

– C-Reactive Protein (CRP): ELISA
– Analytical Sensitivity: < 1 µg/ml
– Intra Assay Precision: 5.12 – 6.84%
– Inter Assay Precision: 11.6 – 12.7%
– Cross-Reactivity: Highly specific for human CRP, zero cross reaction with human monomeric CRP, human pentraxin 3, human pentraxin 2, rat CRP.

STORAGE

Storage requirement : You should store the kit in a refrigerator and ensure that the micro-wells are sealed in dry bags. Any unopened reagents will remain stable until the expiration of the kit itself.

REFERENCES

  1. C-reactive protein and the biology of disease. Immunol Res. (2013) 56 (1): 131-42. Review. Ansar W. and Ghosh S.
  2. Current analytical strategies for C-reactive protein quantification in blood. Clin Chim Acta. (2013) 415:1-9. Review. Algarra M., et al.
  3. C-reactive protein: risk factor, biomarker and/or therapeutic target? Can J Cardiol. (2010) 26 Suppl A: 41A-44A. Review. Genest J.
  4. C-reactive protein and inflammation: conformational changes affect function. Biol Chem. (2015) 396 (11): 1181-97. Review. Wu Y., et al.
  5. Recognition functions of pentameric C-reactive protein in cardiovascular disease. Mediators Inflamm. (2014) 2014: 319215. Review. Agrawal A., et al.
  6. C-reactive protein is expressed and secretion by peripheral blood mononuclear cells. Clin Exp Immunol. (2006) 146(3): 533-539. Haider DG, Leuchten N, Schaller G, et al.

ADDITIONAL INFORMATION

  • Full Name: C-Reactive Protein (CRP) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: < 1.0 µg/ml

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