CRP ELISA Kit (C-Reactive Protein)

INTENDED USE

Human CRP ELISA kit is designed for measuring in vitro quantitative amounts of c-reactive protein (CRP) in human serum, plasma, biological fluids and tissue homogenate samples. This assay has a minimum detection limit of 18.0 pg/ml.

CRP BACKGROUND

C-reactive protein (known as CRP for short) is an acute phase protein that was first discovered and named due to its ability to precipitate the c-polysaccharide of pneumococcus in calcium. It is essentially classified as a superfamily of proteins that are known as pentraxins or pentaxins. These contain cyclic, non-glycosylated structures which are made up of apparently five identical globular non-covalently linked subunits that can be aggregated symmetrically.

During the acute phase response, the concentration of CRP can increase up to 1000-fold within a few hours. Whereas, normally in human biological fluids it is only present in trace amounts (approx. 0.6 mg/L). Certain cytokines (IL-1 alpha, IL-1 beta, TNF-alpha or beta and indirectly by IL-6) are responsible for stimulating it, which results in dramatically enhancing its synthesis by hepatocytes. Among the acute phase proteins, it is the most sensitive, fast-reacting and the easiest to measure, also its catabolism is not affected by the type of inflammation.

Testing is mainly carried out for the medical diagnosis and to monitor and detect health conditions that are normally associated to inflammation. In addition, serum concentration levels are found to significantly increase for infectious or non-infectious inflammations, for necrosis or tissue damage and during the presence of many malignant tumours.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-CRP antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of human c-reactive protein (CRP) using current CRP ELISA kit was 18.75 pg/ml. The dynamic range for this assay is 31.25 – 2,000.0 pg/ml. It is recommended to follow the user manual guide in order to achieve the expected results.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for CRP, no cross reactivity or interference between CRP and analogues was detected.
– Recovery: Serum (85 – 104%), EDTA Plasma (85 – 103%), Heparin Plasma (89 – 99%).
– Linearity: Serum (86 – 104%), EDTA Plasma (814 – 90%), Heparin Plasma (81 – 95%).
– Precison Intra-Assay: CV < 6%.
– Precison Inter-Assay: CV < 6%.
– Stability: Less than 10%.

REFERENCES

1. C-reactive protein and the biology of disease. Immunol Res. (2013) 56 (1): 131-42. Review. Ansar W. and Ghosh S.
2. Is serum CRP level a reliable inflammatory marker in pediatric nephrotic syndrome? Pediatr Nephrol. (2016) 31 (8): 1287-93. Shostak E. et al.
3. Anti-CRP antibodies in systemic lupus erythematosus. Joint Bone Spine. (2010) 77 (5): 384-9. Meyer O.
4. C-reactive protein is expressed and secretion by peripheral blood mononuclear cells. Clin Exp Immunol. (2006) 146(3): 533-539. Haider DG, Leuchten N, Schaller G, et al.

ADDITIONAL INFORMATION

• Full Name: CRP ELISA Kit (C-Reactive Protein)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 18.75 ng/ml

OTHER RELATED ELISA KITS

• hsCRP ELISA Kit
• TNF Alpha ELISA Kit
• Serum Amyloid A ELISA Kit

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