Human serotonin ELISA kit is designed for determining in vitro quantitative concentrations of serotonin (5-hydroxytryptamine) using human serum, plasma, urine and platelets samples. This assay has a minimum detection limit of 6.2 ng/ml.
Serotonin (also called 5-hydroxytryptamine), is a monomer biogenic amine neurotransmitter that is a product of tryptophan metabolism. The majority of it is located in the enterochromaffin cells of the digestive tract (gastrointestinal tract), here it is responsible for regulating movement of the gut. The remainder of the body’s serotonin is synthesized by serotonergic neurons within the central nervous system, these are known to have a number of different functions such as regulating sleep, appetite and mood. Serotonin is also found to have some cognitive functions, such as learning and memory. The modulation of serotonin at synapses junction are thought to be a major action of many classes of pharmacological antidepressants.
Serotonin is mainly metabolised to 5-HIAA and this is chiefly carried out by the liver. Metabolism involves an initial oxidation step by monoamine oxidase in order to form the corresponding aldehyde. This is then followed by oxidation of aldehyde dehydrogenase to 5-HIAA, the indole acetic acid derivative. The latter is predominately excreted by the kidneys.
Serotonin is generally an example of an inhibitory neurotransmitter and medications that are used to increase the availability and uptake are vital in treating depression. It can also be taken up by platelets, which are able to store this hormone. When the platelets attach to a blood clot, this triggers the release serotonin which is vital in acting as a vasoconstrictor and a clotting factor to promote healing.
SEROTONIN ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Serotonin Microtiter Strips: Antigen pre-coated microwell plate.
- Standards (A-F): Concentrations 0, 5, 15, 50, 150, 500, 2,500 ng/ml
- Antiserum: Rabbit anti-serotonin antibody.
- Control 1.
- Control 2.
- Enzyme Conjugate.
- Substrate: Chromogenic Tetramethylbenzidine (TMB).
- Wash Buffer Concentrate (50x).
- Stop Solution.
- Reaction Tubes.
- Acylation Reagent.
- Acylation Buffer.
The minimum sensitivity level of 5-hydroxytryptamine (serotonin) using current serotonin ELISA kit was approximately 6.2 ng/ml. The dynamic range for this assay is 15 – 2,500 ng/ml.
– Serotonin: ELISA
– Analytical Sensitivity: Limit of Detection (LOD) 6.2 ng/ml, Limit of Quantitation (LOQ) 10.2 ng/ml.
– Analytical Sensitivity (Cross Reactivity): Serotonin (100%), Melatonin (0.08%), Tryptamine (0.05%), Histidine (< 0.019%), Tyramine (< 0.018%), 5-Hydroxytryptophane, Phenylalanine, 5- Hydroxyindole acetic acid (< 0.014%).
– Precision Intra Assay: 9.2 – 13.8% (Urine) and 9.7 – 12.6% (Serum)
– Precision Inter Assay: 11.3 – 14.9% (Urine) and 10.4 – 12.4% (Serum)
– Linearity: 88 – 118% (Urine) and 18 – 113% (Serum)
– Recovery: 74 – 105% (Urine) and 89 – 126% (Serum)
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- Full Name: Serotonin ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma, Platelets, Urine
- Sensitivity: 6.2 ng/ml