Glutamate ELISA Kit

INTENDED USE

Human glutamate ELISA kit can be used for measuring in vitro quantitative amounts of glutamate in human serum, plasma (EDTA) and urine. This assay has a sensitivity detection limit of 0.3 µg/ml.

BACKGROUND

Glutamate, in neuroscience is used to describe the anion of glutamic acid in its functions as neurotransmitter that can send signals in the brain and throughout the nerves in the body. It is synthesised from glutamine using glutaminase enzyme within the central nervous system. This process usually occurs in the presynaptic neuron or even in the neighbouring glial cells. It is a major constituent of many proteins and can also serve as a metabolic precursor for the neurotransmitter, through the action of glutamate decarboxylase enzyme.

Glutamate is vital for many bodily functions which includes sending messages for body movement, creation and the management of memory and regulating the human thought process. It is also very important during the development of the brain. Excess levels present in the brain are linked with many neurological diseases for example; Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis and stroke.

Functions of glutamate include: learning and memory (allowing nerve cells to accumulate information through the process of association), source of energy for the brain (especially in times when the levels of glucose is low), chemical messenger (allowing chemical messages to be passed from one nerve to the next) and a signal for pain (high levels of glutamate are linked to elevated levels of pain).

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Adhesive Foil.
• Reaction Plate.
• Extraction Plate.
• Wash Buffer Concentrate (50x).
• Enzyme Conjugate.
• Substrate (Chromogenic).
• Stop Solution.
• Glutamate Microtiter Strips.
• Antiserum – Rabbit anti glutamate antibody.
• Assay Buffer.
• Equalizing Reagent (Lyophilized).
• Standards (A-F) and Controls (1+2) – 0-60 ng/ml.
• D-Reagent – Crosslinking agent in dimethylsulfoxide.
• Q-Buffer.
• Diluent.
• NaOH.

SENSITIVITY

The minimum sensitivity detection limit of human glutamate using current glutamate ELISA kit was approximately 0.3 µg/ml. The dynamic range for this assay is 0.6 – 60 µg/ml.

ASSAY CHARACTERISTICS

– Expected Reference Values: 5 – 100 µmol/g creatinine (Urine) and 2.2 – 30 µg/mL (Serum/Plasma).
– Analytical Sensitivity (Limit of Detection): 0.3 µg/Ml
– Analytical Specificity (Cross Reactivity): Glutamate (100%), Aspartate (0.09%), Glycine, Glutamine, 5-aminovaleric acid, Alanine (< 0.01%).
– Intra Assay Precision: 6.3 – 7.3%
– Inter Assay Precision: 13 – 19%
– Linearity: 83 – 111% (Urine) and 82 – 98% (Serum).
– Recovery: 83 – 105% (Urine) and 96 – 104% (Serum).

REFERENCES

1. Maintaining the presynaptic glutamate supply for excitatory neurotransmission. J Neurosci Res. (2015) 93 (7): 1031-44. Review. Marx M.C., et al.
2. The glutamate-hypothesis in ALS: pathophysiology and drug development. Curr Med Chem. (2014) 21 (31): 3551-75. Review. Blasco H., et al.
3. Multiple sclerosis and glutamate excitotoxicity. Rev Neurosci. (2013) 24 (1): 71-88. Review. Kostic M., et al.

ADDITIONAL INFORMATION

• Full Name: Glutamate ELISA Kit
• Reactivity: Human
• Sample Type: Plasma (EDTA), Serum, Urine
• Sensitivity: 0.3 µg/ml

OTHER RELATED ELISA KITS

• Histamine ELISA Kit
• Serotonin ELISA Kit
• Tryptophan ELISA Kit