Histamine ELISA Kit
INTENDED USE
Human histamine ELISA kit is a procedure designed for detecting in vitro quantitative levels of histamine and beta imidazolethylamine in human plasma (EDTA, heparin) and urine samples. This assay has a minimum sensitivity detection limit of 0.12 ng/ml (P) and 0.30 ng/ml (U).
BACKGROUND
Histamine or beta imidazolethylamine, in the human body is found in almost every tissue where it is stored predominately in the form of granules of tissue mast cells. Blood cells which are known as basophils are also harbouring histamine-containing granules. It has many important roles after it has been released from the granules and these include aiding contraction of smooth muscles tissues of the stomach, lungs and uterus; the stimulation of gastric acid secretion within the stomach; acceleration of the hearth rate and increasing permeability whilst lowering blood pressure. It serves as a neurotransmitter where it is able to carry chemical messages between nerve cells.
Histamine functions through binding to histamine receptors which are located on the surface of specific cells. There are four kinds of binding receptors which are called; H1, H2, H3 and H4. The activity is inhibited by a number of drugs which are called antihistamines and these are able to block them from binding to its receptors. Conventional anti-histamine have been useful in treating allergies to block H1 receptors (called H1 antagonists) examples include Tagamet (cimetidine) which has the ability to block gastric acid secretion and this has been vital in aiding to heal peptic ulcers.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Adhesive Foil.
- Wash Buffer (50x Concentrate).
- Reaction Plate: 1 x 96 well plate, empty.
- Enzyme Conjugate.
- TMB Substrate.
- Stop Solution.
- Histamine Microtiter Strips.
- Standards (A-F): Concentrations 0, 0.5, 1.5, 5, 15, 50 ng/ml.
- Control 1.
- Control 2.
- Histamine Antiserum.
- Acylation Solvent.
- Acylation Buffer.
- Acylation Reagent (Lyophilized).
SENSITIVITY
The minimum sensitivity detection limit of human histamine (beta imidazolethylamine) using current histamine ELISA kit was approximately 0.30 ng/ml (U) and 0.12 ng/ml (P). The dynamic range for this assay is 0.5 – 50 ng/ml.
ASSAY CHARACTERISTICS
– Analytical Sensitivity (Limit of Quantitation, LOQ): 0.29 ng/mL (Plasma) and 0.96 ng/mL (Urine).
– Analytical Sensitivity (Limit of Detection, LOD): 0.18 ng/mL (Plasma) and 0.22 ng/mL (Urine).
– Analytical Specificity (Cross Reactivity): Histamine (100%), 3-Methyl-Histamine (0.1%), Tyramine (0.01%), L-Phenylalanine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin (< 0.001%).
– Intra Assay Precision: 13 – 15% (Urine) and 12 – 16% (Plasma).
– Inter Assay Precision: 11 – 14% (Urine) and 8 – 25% (Plasma).
– Linearity: 90 – 124% (Urine) and 85 – 106% (Plasma).
– Recovery: 101 – 119% (Urine) and 78 – 89% (Plasma).
REFERENCES
- Developmental roles of brain-histamine. Trends Neurosci. (2014) 37 (3): 159-68. Review. Panula P., et al.
- Histamine and H1-antihistamines: celebrating a century of progress. J Allergy Clin Immunol. (2011) 128 (6): 1139-1150.e4. Review. Simons F.E. and Simons K.J.
- The neglected role of histamine in Alzheimer’s disease. Am J Alzheimers Dis Other Demen. (2013) 28 (4): 327-36. Review. Naddafi F. and Mirshafiey A.
ADDITIONAL INFORMATION
- Full Name: Histamine ELISA Kit
- Reactivity: Human
- Sample Type: Plasma (Heparin, EDTA), Urine
- Sensitivity: 0.12 ng/ml (P) and 0.30 ng/ml (U)
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