Procalcitonin ELISA Kit (PCT)

Full Name: Procalcitonin ELISA Kit (PCT)
Reactivity: Human
Sample Type: Serum, Plasma, Biological Fluids, Tissue Homogenates
Sensitivity: 18.75 pg/ml

INTRODUCTION

The thyroid C-cells are principally responsible for the production and secretion of the peptide hormone procalcitonin (PCT). Its structure is composed of 116 amino acids. Its synthesis is triggered by bacterial infection or inflammation. The main function is to regulate the production of other hormones, such as calcitonin. It as been used as a useful biological marker during the investigation of bacterial infections and sepsis. Elevated levels were found to be present in blood samples which was associated with bacterial infections. Early diagnosis of sepsis is critical for effective treatment and PCT levels can help healthcare providers determine the likelihood of sepsis and make timely treatment decisions.

Procalcitonin measurement in blood has been proposed to guide antibiotic therapy, with the goal of reducing unnecessary use of antibiotics. High levels are indicative of a persistent infection and antibiotics treatment may need to be extended. Whereas, low levels, indicate that an infection is resolving and antibiotics treatment can be discontinued. Circulating procalcitonin may be derived from plasma or from bone marrow-derived granulocytes. The levels are influenced by; age, gender, diseases (such as diabetes) and diet (such as milk consumption).

INTENDED USE

Human procalcitonin ELISA kit is designed for detecting concentrations of PCT (procalcitonin) present in human in plasma, serum, biological fluids and tissue homogenates. This assay has a sensitivity detection limit of 18.75 pg/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with PCT
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

The following procalcitonin ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml.
Reactivity: Human
Sensitivity: 18.75 pg/ml
Range: 31.25 – 2000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for PCT, no cross reactivity or interference between PCT and analogues was detected.
– Recovery: Serum (93 – 100%), EDTA Plasma (89 – 103%), Heparin Plasma (88 – 103%).
– Linearity: Serum (87 – 99%), EDTA Plasma (85 – 97%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Procalcitonin Monitoring as a Guide for Antimicrobial Therapy: A Review of Current Literature. (2018) 38 (5): 569-581. Covington E.W., et al.
2. Sepsis in Trauma: A Deadly Complication. Arch Med Res. (2021) 52 (8): 808-816. Mas-Celis F., et al.
3. The role of procalcitonin in the management of pleural infection. Curr Opin Pulm Med. 2018 Jul;24(4):380-383. de Fonseka D. and Maskell N.A.

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