Osteopontin N-Half ELISA Kit (Mouse)
INTENDED USE
Mouse osteopontin N-half ELISA kit can only be used to specifically quantitatively detect the mouse osteopontin N-terminal half (OPN N-Half) using urine, cell culture media and EDTA plasma samples. This assay has a minimum sensitivity detection limit of 5.63 pmol/l.
BACKGROUND
Osteopontin (OPN) is a calcium-binding highly acidic glycosylated phosphoprotein, it contains a sequence of Arg-Gly-Asp (RGD) amino acids which can also be found in other protein family members such as fibronectin, integrin and vitronectin. The molecular weight of this protein can range between 44 and 66 kDa depending on its glycosylation or phosphorylation state. Osteopontin can be secreted by osteoblasts, kidney tubule cells, activated T-cells and vascular smooth muscle cells. The following procedure has be designed to specifically detect the N-terminal fragment of thrombin cleaved mouse OPN (OPN N-Half) and this assay will not detect the uncleaved OPN.
MOUSE OSTEOPONTIN N-HALF ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Pre-Coated Plate: Anti-OPN N-Half (34E3) mouse IgG.
- Labelled Antibody (30x).
- Standard: Recombinant mouse OPN N-Half.
- EIA Buffer.
- Solution For Labelled Antibody.
- TMB Chromogen.
- Wash Buffer (40x Concentrated).
- Stop Solution.
SENSITIVITY
The minimum sensitivity detection limit of Osteopontin N-terminal half (Osteopontin N-Half) using current mouse OPN N-Half ELISA kit is 5.63 pmol/l. The dynamic range for this assay is 25.0 – 41,600.0 pmol/l.
ASSAY CHARACTERISTICS
– Mouse Osteopontin N-Half (OPN N-Half): ELISA
– Titer Assay: 86.2 – 106.7%
– Added Recovery Assay: 85.4 – 96.1%
– Intra – Assay: 8.3 – 10.0%
– Inter – Assay: 7.5 – 9.1%
– Sensitivity: 5.63 pmol/L
REFERENCES
- Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis. J Rheumatol. (2011) 38 (1): 129-34. Hasegawa M., et al.
- The osteopontin transgenic mouse is a new model for Sjögren’s syndrome. Clin Immunol. (2015) 157 (1): 30-42. Husain-Krautter S., et al.
- Osteopontin level in synovial fluid is associated with the severity of joint pain and cartilage degradation after anterior cruciate ligament rupture. PLoS One. (2012) 7 (11): e49014. Yamaga M., et al.
- Osteopontin – a multi-modal marker and mediator in atherosclerotic vascular disease. Atherosclerosis. (2014) 236 (2): 327-37. Review. Wolak T.
- Cleaved Form of Osteopontin in Urine as a Clinical Marker of Lupus Nephritis. PLoS One. (2016) 11 (12): e0167141. Kitagori K., et al.
ADDITIONAL INFORMATION
- Full Name: Osteopontin N-Half ELISA Kit (Mouse)
- Reactivity: Mouse
- Sample Type: Cell Culture Media, Plasma, Urine
- Sensitivity: 5.63 pmol/l
