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Rat Osteopontin ELISA Kit (OPN)

Full Name: Rat Osteopontin ELISA Kit (OPN)
Reactivity: Rat
Sample Type: Urine, EDTA Plasma, Cell Culture Media
Sensitivity: 0.01 ng/ml

BACKGROUND

Cell-cell adhesion, tissue remodelling and wound healing all depend on osteopontin. The molecular weight of OPN can range from 44-66 kDa, which makes it an extremely versatile protein. This wide range of molecular weights allows researchers to study the different roles it plays in different biological contexts and helps them explore the potential therapeutic implications of this protein. The OPN gene is located on chromosome 15q14-15.3. It is also expressed in some tumor cells and tissue regenerating cells such as fibrocytes and mast cells. It plays a part in bone remodelling and wound healing as an extracellular matrix protein by encouraging the growth of new blood vessels and vascular networks that deliver nutrients to the site of sickness or injury. There are multiple mechanisms through which OPN promotes angiogenesis such as increasing endothelial cell proliferation, migration and tube formation.

Osteopontin (OPN) is a incredibly glycosylated acidic calcium binding phosphoprotein, that’s launched through vascular easy muscle cells, kidney tubule cells, osteoblasts and activated T-cells. In neurons, this protein binds to its own promoter region from exon 10 to exon 13 via its N-terminal sequence. A substantial amount of study examining the involvement in neurodegenerative illnesses like Alzheimer’s disease has been inspired by the discovery that osteopontin depletion increases synaptic degeneration in the hippocampus (AD). Its ability to attach to the ubiquitin ligase complex and mediate proteasome degradation is one way it can contribute to AD. Osteopontin is also known as CD206, ROD1 (receptor for Osteopontin) or P2Y2 receptor 1.

INTENDED USE

Rat osteopontin ELISA kit is intended for measuring quantitative concentrations of osteopontin (OPN) using rat EDTA plasma, cell culture media or urine samples. This assay has a minimum sensitivity detection limit of 0.01 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Pre-Coated Plate: Anti-rat OPN (0-17) rabbit IgG.
  • Labelled Antibody (30x).
  • Standard.
  • Solution For Labelled Antibody.
  • EIA Buffer.
  • TMB Chromogen.
  • Wash Buffer (40x Concentrated).
  • Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of osteopontin (OPN) using current rat osteopontin ELISA kit is 0.01 ng/ml. The dynamic range for this assay is 0.07 – 4.75 ng/ml.

ASSAY CHARACTERISTICS

– Titer Assay: 92.9 – 108.8%
– Added Recovery Assay: 81.9 – 114.7%
– Intra – Assay: 4.5 – 5.5%
– Inter – Assay: 3.2 – 5.6%
– Specificity: Rat OPN (100%), Human OPN (≦0.1%), Mouse OPN (≦0.1%)
– Sensitivity: 0.01 ng/mL

REFERENCES

  1. Osteopontin_synthesis and localization along the human nephron. J Am Soc Nephrol. (2002) 13 (5): 1210-8. Verhulst A., et al.
  2. High Concentration of Calcium Stimulates Calcium Oxalate Crystal Attachment to Rat Tubular Epithelial NRK Cells Through Osteopontin. Urology. (2015) 86 (4): 844. e1-5. Xi Q., et al.
  3. The role of transglutaminase 2 and osteopontin in matrix protein supplemented microencapsulation of marrow stromal cells. Biomaterials. (2010) 31 (35): 9256-65. Hakimzadeh N., et al.
  4. A combination of sinomenine and methotrexate reduces joint damage of collagen induced arthritis in rats by modulating osteoclast-related cytokines. Int Immunopharmacol. (2014) 18 (1): 135-41. Sun Y., et al.

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