Osteocalcin ELISA Kit (OST)

INTENDED USE

Human osteocalcin ELISA kit is a reliable quantitative procedure for measuring osteocalcin (OST) in human serum. This assay has a sensitivity detection limit of 0.08 ng/ml.

BACKGROUND

Osteocalcin (OST) which is also called bone Gla protein (B.G.P) is a 49 amino-acids peptide which contains three residues of gamma carboxyl glutamic acids and is 5800 Da in molecular weight. It is synthesised in the bone by the osteoblasts where after its production it is partly incorporated in the bone matrix, the rest of it is found to be circulating in blood.

There are a large number of publications which show a direct correlation between the rate of bone formation and the levels of circulating osteocalcin but the exact physiological function is yet still unclear. However, it has been reported to have minimum effect on bone density and mineralisation. Instead, osteocalcin controls many different physiological processes within the endocrine system for example brain development, male fertility, cognition, glucose homeostasis and exercise capacity.

In various clinical applications determining the levels of human osteocalcin is helpful in many cases for example when:
(a). monitoring bone metabolism during the perimenopause and post-menopause.
(b). monitoring bone metabolism in patients that have; growth hormone deficiency, chronic renal failure, hypothyroidism and hyperthyroidism.
(c). identifying women that are at potential risk of developing osteoporosis.
(d). monitoring bone metabolism during treatment of premenopausal women with LH-RH agonists and hormone replacement therapy.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiterplate: Coated with anti-osteocalcin antigen.
• Conjugate Buffer.
• Conjugate: HRP labelled conjugated monoclonal antibodies.
• Calibrators 1-5: Concentrations 0, 1.56, 4.10, 12.7, 31.5, 75 ng/ml.
• Wash Solution (200x Concentrated).
• Controls 1 and 2: Containing anti human osteocalcin.
• Chromogenic Solution: Tetramethylbenzydine (TMB).
• Stop Solution: HCl (1N).

SENSITIVITY

The minimum sensitivity detection limit of human osteocalcin (OST) using current osteocalcin ELISA kit was approximately 0.08 ng/ml. The dynamic range for this assay is 0 – 75.0 ng/ml.

ASSAY CHARACTERISTICS

– Detection Limit: 0.08 ng/ml
– Specificity: Highly specific for intact human osteocalcin.
– Intra-Assay Precision: 3.1 – 4.7%
– Inter-Assay Precision: 3.5 – 5.6%
– Recovery (Accuracy): 97 – 111%
– Dilution Test: 3.7 – 30.8 ng/ml

REFERENCES

1. Osteocalcin: a potential marker of metastatic bone disease and response to treatment. Eur. J. Cancer Clin. Oncol.(1988) 24,1211-1217. R.E. Coleman, et al.
2. Osteocalcin: Diagnostic Methods and Clinical Applications. Crit. Rev. Clin. Lab. Sci. (1991) 28, 4, 287-335. M.J. Power and P.F. Fottrell.
3. An evaluation of serum osteocalcin in Paget’s disease of bone and its response to diphosphonate treatment. Arthritis and Rheumatism (1988) 31, 9, 1142-1147. L.A. Coulton, et al.
4. Serum BGP: a specific marker for bone formation in postmenopausal osteoporosis. The Lancet (1984) 1091-1093. J.P. Brown et al.

ADDITIONAL INFORMATION

• Full Name: Osteocalcin ELISA Kit (OST)
• Reactivity: Human
• Sample Type: Serum
• Sensitivity: 0.08 ng/ml

OTHER RELATED ELISA KITS

• Human Osteopontin ELISA Kit
• Intact PTH ELISA Kit
• Calcitonin ELISA Kit