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Calcitonin ELISA Kit

INTENDED USE

Human calcitonin ELISA kit is a reliable quantitative procedure for measuring calcitonin (CT) in human serum or plasma. This assay has a sensitivity detection limit of 1.3 pg/ml.

BACKGROUND

Calcitonin (CT) is a 32 amino acid peptide hormone which is produced and secreted from the para-follicular C-cells of the thyroid gland and it’s function is to oppose the action of parathyroid hormone (PTH), this helps to regulate bloods phosphate and calcium levels. Calcitonin function is carried out through the inhibition of osteoclast cell activity, these cells are primarily responsible for breakdown of bone. This process causes the amount calcium that is present in the blood to be reduced and the secretion of this hormone is directly controlled by the level of calcium that is present in the blood. Various forms of calcitonin are known to be detected in blood samples, for example monomer, dimer, oxidized monomer, precursor of CT and higher molecular weight forms. The concentrations of these peptides is found to vary depending on the clinical status, renal function and normal or ectopic production.

Medullary carcinoma of the thyroid (MTC) is one of the most prominent clinical syndrome that is linked to a disordered hypersecretion of calcitonin, even though it is rarely representing 5-10% of all thyroid cancers, it has the potential to be quite fatal in many cases. The use of serum samples in particular have been instrumental in diagnosing the presence of MTC, it is able to provide exceptional degree of specificity and accuracy. It is also recommend that the monomer form of calcitonin is detected.

CALCITONIN ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Coated with anti-CT monoclonal antibodies.
  • HRP Labelled Conjugate.
  • Conjugate Buffer.
  • Calibrators (0-5): Concentrations 0, 10, 50, 100, 200 400 pg/ml.
  • Calcitonin Free Human Serum: To be used for samples dilution with thymol.
  • Borate Buffer (Serum Free).
  • Wash Solution (200x Concentrated).
  • Control 1 (High).
  • Control 2 (Low).
  • TMB Chromogenic Solution.
  • Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of calcitonin (CT) using current human calcitonin ELISA kit was approximately 1.3 pg/ml. The dynamic range for this assay is 7.5 – 573.0 pg/ml.

ASSAY CHARACTERISTICS

– Calcitonin (CT): ELISA
– Detection Limit: 0.7 pg/ml
– Specificity: At concentrations up to 100 ng/ml, none of the following hormones showed significant interference: CGRP, Salmon Calcitonin, PDN 21, Procalcitonin N terminal.
– Intra-Assay Precision: 1.7 – 3.9%
– Inter-Assay Precision: 4.9 – 6.0%
– Recovery Test: 99 – 105%
– Hook Effect: A sample spiked with CT up to 480000 pg/ml gives higher OD’s than the last calibrator point.

REFERENCES

  1. Plasma calcitonin as a marker of disease activity in patients with small cell carcinoma of the lung. J. Clin. Endocrinol. Metab. (1981) 53, 3: 602-606. Wallach S.R., Royston I, Taetle R., Wohl H. and Deftos L.
  2. Abnormal calcitonin basal levels and pentagastrin response in patients with chronic renal failure on maintenance hemodialysis. Eur. J. Endocrinol. (1995) 132, 1, 75-81. Nicoli P., et al.
  3. Calcitriol corrects deficient calcitonin secretion in the Vit. D deficient elderly. J. Bone Miner Res. (1994) 9, 1, 53-57. Quesada J.M., et al.
  4. Influence of age and sex on plasma calcitonin in human beings. N. Engl. J. Med. (1980) 302: 1351-1353. Deftos, L.J., et al.
  5. Estimates of circulating monomeric calcitonin: physiological studies in normal and thyroidectomized man. J. Clin. Endocrinol. Metab. (1983) 57: 897. Body J.J. and Heath III, H.

ADDITIONAL INFORMATION

  • Full Name: Calcitonin ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.3 pg/ml

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