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Nucleosome Ab ELISA Kit

INTENDED USE

Human nucleosome antibody ELISA kit can be used for measuring in-vitro quantitative levels of IgG auto-antibodies to nucleosome (nucleosome IgG, nucleosome Ab, anti-nucleosome) in human serum and plasma. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.

BACKGROUND

Nucleosomes are repeating units which are fundamental to eukaryotic chromatin and they are folded through a successive series of higher order structures in order to form the chromosome. This process causes the DNA to be compact and creates an added layer of regulatory control. The core particle of the nucleosome is approx. 146 base pairs of DNA that is wrapped around a histone octomer which contains 2 copies of H2A, H2B, H3 and H4 (known as the core). They are also thought to carry epigenetically inherited information which is the result of covalent modifications of their core histones.

NUCELOSOME AB ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Divisible Microplate: Consisting of 12 modules of 8 wells, nucleosomes Ab are bound to microwells.
  • Calibrator A-F: Concentration 0, 12.5, 25, 50, 100, 200 U/ml.
  • Control Positive and Control Negative: Containing ,anti-nucleosome in a serum/buffer matrix.
  • Sample Buffer PD (5x).
  • Enzyme Conjugate: Contains anti-human IgG antibodies, HRP labelled.
  • TMB Substrate.
  • Stop Solution: Contains acid.
  • Wash Buffer (50x Concentrated).
  • Instruction For Use.
  • Certificate Of Analysis.

SENSITIVITY

The minimum detection sensitivity level of IgG antibody to nucleosome (nucleosome IgG, nucleosome Ab, anti-nucleosome) using current nucleosome antibody ELISA kit was 0.5 U/ml. The dynamic range for this assay is 12.5 – 200.0 U/ml.

ASSAY CHARACTERISTICS

– Nucleosome Antibody: ELISA
– Sensitivity: 1.0 U/ml
– Results Interpretation: Serum samples from healthy blood donors displayed a cut-off 20 U/ml.
– Parallelism: Displayed linearity over the full measuring range.
– Intra Assay: 3.1 – 6.4%
– Inter Assay: 5.2 – 12.4%
– Specificity: Recognizes only autoantibodies directed against nucleosomes.
– Interfering Substances: None

REFERENCES

  1. Are anti-nucleosome antibodies a better diagnostic marker than anti-dsDNA antibodies for systemic lupus erythematosus? A systematic review and a study of metanalysis. Autoimmun Rev. (2012) 12 (2): 97-106. Review. Bizzaro N., et al.
  2. Nucleosome autoantibodies. Clin Chim Acta. (2006) 366 (1-2): 48-60. Review. Decker P.
  3. Anti-chromatin (anti-nucleosome) antibodies. Lupus. (2006) 15 (7): 408-11. Review. Gómez-Puerta J.A., et al.
  4. Review: antinucleosome antibodies: a critical reflection on their specificities and diagnostic impact. Arthritis Rheumatol. (2014) 66 (5): 1061-9. Rekvig O.P., et al.

ADDITIONAL INFORMATION

  • Full Name: Nucleosome Ab ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: 0.5 U/ml

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