Histone Ab ELISA Kit

INTENDED USE

Human histone Ab ELISA kit is a method for measuring in-vitro quantitative amounts of IgG autoantibodies against histone antibody (anti-histone, histone Ab, anti-histone IgG) in human serum or plasma. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

BACKGROUND

Histones auto-antibodies are highly conserved in many species and are the major basic protein that is found in chromatin. There are currently 5 different classes H1, H2, H2b, H3 and H4, all of these range in molecular weight from 11-23 kD. They contain arginine and lysine basic amino acids and are antinuclear antibodies (ANA) which are associated with lupus.

The presence or even an absence of a particular antinuclear antibodies can be a useful aid in determining a specific disorder. Many drugs are associated with drug-induced lupus in some people such as; hydralazine, penicillamine, isoniazid, quinidine, procainamide and minocycline. However, majority of anti-histone antibodies in drug induced lupus erythematosus are of temporary character and tend to disappear within a few months after treatment.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Divisible Microplate: Consisting of 12 modules of 8 wells, highly purified total histones are bound to microwells.
• Calibrator A-F: Concentration 0-200 U/ml, made up from human histone antibody.
• Control Positive and Control Negative: Containing anti-histone antibody in a serum/buffer matrix.
• Sample Buffer P (5x).
• Enzyme Conjugate: Contains anti-human IgG antibodies, HRP labelled.
• Substrate: Tetramethylbenzidin (TMB).
• Stop Solution: Contains acid.
• Wash Buffer (50x).
• Instruction for Use.
• Certificate of Analysis.

SENSITIVITY

The minimum detection sensitivity level of histone IgG auto-antibodies (histone Ab, anti-histone IgG, anti-histone) using current histone ELISA kit was 1.0 U/ml. The dynamic range for this assay is 12.5 – 200.0 U/ml.

REFERENCES

1. The complex role of DNA, histones and HMGB1 in the pathogenesis of SLE. Autoimmunity. (2014) 47 (8): 487-93. Review. Pisetsky D.S.
2. Autoantibodies to nucleosomes and histone-DNA complexes. Methods. (1997) 11 (1): 36-43. Monestier M.
3. The spectrum of anti-chromatin/nucleosome autoantibodies: independent and interdependent biomarkers of disease. J Immunol Res. (2014) 2014: 368274. Review. Mehra S. and Fritzler M.J.
4. Antibodies to histones in systemic lupus erythematosus and drug-induced lupus syndromes. Rheum Dis Clin North Am. (1992) 18 (2): 415-36. Review. Monestier M. and Kotzin B.L.
5. Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv Immunol. (1982) 33: 167-240. Review. Tan E.M.

ADDITIONAL INFORMATION

• Full Name: Histone Ab ELISA Kit
• Reactivity: Human
• Sample Type: Serum, Plasma
• Sensitivity: 1.0 U/ml

OTHER RELATED ELISA KITS

• Nucleosome ELISA Kit
• Anti-dsDNA Screen ELISA Kit
• ANA Profile ELISA Kit

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