Lactoferrin Ab ELISA Kit (LF)

Full Name: Lactoferrin Ab ELISA Kit (LF)
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 0.5 U/ml

BACKGROUND

Lactofеrrin (LF) also callеd lactotransfеrrin (LTF) is an iron binding protеin which bеlongs to thе transfеrrin family, it is involvеd in thе innatе immunе systеm. It mainly rеsidеs in spеcific granulеs of thе polymorph nuclеar nеutrophil lеukocytеs (PMN) and can bе prеsеnt during sеcrеtions for еxamplе in tеars, saliva or milk.  It is commonly producеd from milk, whеrе human colostrum is known to contain thе grеatеst concеntration, aftеr that it is human milk followеd by cow milk.

Auto-antibodiеs against lactofеrrin bеlong to thе pANCA group and thеsе arе found to bе at еlеvatеd lеvеls in patiеnts with ulcеrativе colitis, primary sclеrosing cholangitis and rhеumatoid vasculitis. During many activе inflammatory disеasе, thе highеr sеrum concеntrations of lactofеrrin can bе mеasurеd. Somе of thе othеr rolеs includе stimulation of thе immunе systеm, prеvеntion of tissuе damagе associatеd to aging, prеvеntion of many cancеrs, promotion of hеalthy intеstinal bactеria and rеgulation of how iron is procеssеd by thе body.

INTENDED USE

Human lactoferrin Ab ELISA kit is intended for analysing in-vitro quantitative amounts of IgG auto-antibodies to lactoferrin (lactoferrin Ab, LF, lactotransferrin, LTF, lactoferrin IgG) in plasma or serum. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Divisible Microplate: Consisting of 12 modules of 8 wells, highly purified lactoferrin Ab is bound to microwells.
• Calibrators A-F: Concentration 0, 6.3, 12.5, 25, 50, 100 U/ml.
• Control Positive and Control Negative: Containing human lactoferrin antibody in a serum/buffer matrix.
• Sample Buffer P (5x).
• Enzyme Conjugate: Contains anti-human IgG antibodies, HRP labelled.
• TMB Substrate.
• Stop Solution: Contains acid.
• Wash Buffer (50x).

SENSITIVITY

The minimum detection sensitivity level of lactoferrin IgG autoantibodies (LTF, lactoferrin Ab, lactotransferrin, LF, lactoferrin IgG) using current lactoferrin ELISA kit was 0.5 U/ml. The dynamic range for this assay is 6.3 – 100.0 U/ml.

ASSAY CHARACTERISTICS

– Results Interpretation: Serum samples from healthy blood donors displayed normal < 10 U/ml, elevated > 10 U/ml.
– Sensitivity: 0.5 U/ml
– Specificity: Highly specific only for autoantibodies directed against Lactoferrin Ab. No cross reactivities to the other ANCA antigens have been observed.
– Parallelism: Displayed linearity over the full measuring range.
– Interfering Substances: None

REFERENCES

1. Immunomodulatory effects of lactoferrin. Acta Pharmacol Sin. (2014) 35 (5): 557-66. Review. Siqueiros-Cendón T., et al.
2. Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine. Molecules. (2016) 21 (6). pii: E752. Review. Bruni N., et al.
3. Lactoferrin: structure, function and applications. Int J Antimicrob Agents. (2009) 33 (4): 301.e1-8. Review. González-Chávez S.A., et al.
4. LF immunomodulatory strategies: mastering bacterial endotoxin. Biochem Cell Biol. (2012) 90 (3): 269-78. Review. Latorre D., et al.
5. Lactoferrin, a key molecule in immune and inflammatory processes. Biochem Cell Biol. (2012) 90 (3): 252-68. Review. Legrand D.
6. Lactoferricin: a lactoferrin-derived peptide with antimicrobial, antiviral, antitumor and immunological properties. Cell Mol Life Sci. (2005) 62 (22): 2588-98. Review. Gifford J.L., et al.

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