NLRP3 ELISA Kit (Nod Like Receptor Pyrins-3)

INTENDED USE

Human NLRP3 ELISA kit can measure concentrations of nod like receptor pyrins-3 (NLRP3) present is plasma, serum, biological fluids or tissue homogenate samples.

INTRODUCTION

NLRP3, also known as NOD receptor pyrin domain containing 3 is a protein complex that plays a role, in the body’s immune responses. It belongs to a group of receptors called NOD receptors, which are found inside cells and can recognize signals associated with pathogens and potential harm.

Specifically, NLRP3 is a part of the inflammasome, which is a complex made up of multiple proteins that helps activate pro inflammatory cytokines like interleukin 1beta. When various signals such as toxins ATP (adenosine triphosphate) or crystalline substances trigger it comes together with proteins to form the inflammasome complex. This triggers caspase 1 activation leading to the conversion of pro IL 1beta into its active form. The release of IL 1beta then starts a response to fight against pathogens or tissue damage. However excessive activation of NLRP3 can cause inflammation. Has been associated with different autoimmune and inflammatory diseases.

While NLRP3 is primarily found in cells like macrophages, neutrophils, and dendritic cells it can also be present in other cell types such as epithelial cells, osteoblasts (bone forming cells) and neurons. This widespread distribution allows NLRP3 to detect danger signals indicating invasion or cellular damage, throughout the body.

Besides pathogens and molecules disruptions, in the release of potassium, calcium signaling, malfunctioning mitochondria and oxidative stress have the potential to activate NLRP3. This highlights the role, as a detector of responding to various internal and external threats.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-NLRP3 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this NLRP3 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.312, 0.625, 1.25, 5, 10, 20 ng/ml.
Reactivity: Human
Sensitivity: 0.469 ng/ml
Range: 0.781 – 50 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for NLRP3, no cross reactivity or interference between NLRP3 and analogues was detected.
– Recovery: Serum (86 – 98%), EDTA Plasma (85 – 104%), Heparin Plasma (87 – 102%).
– Linearity: Serum (85 – 99%), EDTA Plasma (91 – 97%), Heparin Plasma (80 – 95%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. NLRP3 inflammasome and its inhibitors: a review. Front Pharmacol. (2015) 6: 262. Shao B.Z., et al.
2. The NLRP3-inflammasome as a sensor of organelle dysfunction. J Cell Biol. (2020) 219 (12): e202006194. Seoane P.I., et al.
3. Naringenin attenuates non-alcoholic fatty liver disease by down-regulating the NLRP3/NF-κB pathway in mice. Br J Pharmacol. (2020) 177 (8): 1806-1821. Wang Q., et al.
4. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature. (2016) 530 (7590): 354-7. He Y., et al.

ADDITIONAL INFORMATION

• Full Name: NLRP3 ELISA Kit (Nod Like Receptor Pyrins-3)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 0.469 ng/ml

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