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Myeloperoxidase (MPO) ELISA Kit

INTENDED USE

Human MPO ELISA kit is designed for determining in vitro quantitative amounts of myeloperoxidase (MPO) present in human plasma, serum, tissue homogenate and other biological fluids. This assay has a minimum detection limit of 0.938 ng/ml.

BACKGROUND

Myeloperoxidase (MPO) is a cationic homodimer which is made up of two 15-kDa light chains and two variable-weight glycosylated heavy chains that are bound to a prosthetic heme group. It is essentially a lysosomal protein which is stored in azurophilic granules of neutrophil and then can be released into the extracellular space during the process of degranulation. MPO is abundantly expressed in neutrophils granulocytes and is secreted when they are activated, it is a mammalian phagocyte hemoprotein which can function in mediating host defence reactions.

Intra-nuclear MPO can also help to protect DNA against damage as a result from oxygen radicals that are produced during myeloid cell function and maturation. Myeloperoxidase is a member of the XPO subfamily of peroxidases where it has the ability to produce hypochlorous acid from hydrogen peroxide and chloride anion during the neutrophil’s respiratory burst. Both hypochlorous acid and tyrosyl radical are cytotoxic and they are used by the neutrophil to aid in killing bacteria and many other pathogens. However, there is also evidence that hypochlorous acid is able to result in oxidative damage within host tissue.

Where it has been found that MPO oxidation of apoA-I can lead to a reduction in HDL-mediated inhibition of inflammation and apoptosis. Other functions of MPO include mediating protein nitrosylation   and the formation of dityrosine crosslinks and 3-chlorotyrosine.

MPO ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti MPO antibody.
  • Standards: Concentrations 0, 1.56, 3.125, 6.25, 12.5, 25, 50, 100 ng/ml, made up from lyophilized recombinant human myeloperoxidase.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled MPO Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of myeloperoxidase (MPO) using current human MPO ELISA kit was 0.938 ng/ml. The dynamic range for this assay is 1.563 – 100 ng/ml.

ASSAY CHARACTERISTICS

– Myeloperoxidase (MPO): ELISA
– Specificity: Highly specific for MPO, no cross reactivity or interference between MPO and analogues was detected.
– Recovery: Serum (94 – 104%), EDTA Plasma (89 – 100%), Heparin Plasma (86 – 97%).
– Linearity: Serum (98 – 105%), EDTA Plasma (91 – 100%), Heparin Plasma (84 – 94%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Myeloperoxidase: a front-line defender against phagocytosed microorganisms. J Leukoc Biol. (2013) 93 (2): 185-98. Review. Klebanoff S.J., et al.
  2. Natural and disease associated anti-myeloperoxidase (MPO) autoantibodies. Autoimmun Rev. (2008) 7 (6): 421-5. Review. Guilpain P., et al.
  3. Human myeloperoxidase in innate and acquired immunity. Arch Biochem Biophys. (2010) 500 (1): 92-106. Review. Arnhold J. and Flemmig J.
  4. Coexistence of anti-glomerular basement membrane antibodies and myeloperoxidase-ANCAs in crescentic glomerulonephritis. Am J Kidney Dis. (2005) 46 (2): 253-62. Review. Rutgers A., et al.
  5. A retrospective study on the outcomes of MPO-ANCA-associated vasculitis in dialysis-dependent patients. Mod Rheumatol. (2016) 26 (1): 110-4. Hasegawa M., et al.
  6. Myeloperoxidase: a target for new drug development? Br J Pharmacol. (2007) 152 (6): 838-54. Review. Malle E., et al.

ADDITIONAL INFORMATION

  • Full Name: Myeloperoxidase (MPO) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Tissue Homogenates, Plasma, Biological Fluids
  • Sensitivity: 0.938 ng/ml

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