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Amyloid Precursor Protein (APP) ELISA Kit

INTENDED USE

Human amyloid precursor protein ELISA kit is a procedure designed for determining in vitro quantitative levels of amyloid precursor protein (APP) in human serum, plasma, tissue homogenates and other biological fluids. This assay has a minimum detection limit of 0.563 ng/ml.

BACKGROUND

Amyloid precursor protein (APP) is an essential integral membrane functioning protein which can be found expressed in a number of different tissues and in particular is concentrated within the synapses of neurons. In humans it is encoded by the APP gene which is known to be located on chromosome 21, it is approx. 290 kb and consists of 18 exons. It is thought to have vital roles in the regulation of neural plasticity, synapse formation and iron export. It is also a precursor molecule which can form beta amyloid (Aβ) upon proteolysis and these polypeptides are responsible for forming the main components of amyloid plaques found in the brains of patients suffer with Alzheimer’s disease. The following human APP ELISA kit in intended for analysing various diagnostic applications.

There are been many publication indicating that APP can undergoe extensive post-translational modification such as phosphorylation, glycosylation, tyrosine sulfation, sialylation and many different types of proteolytic processes in order to generate peptide fragment. Often it is found to be cleaved by proteases (e.g. alpha-secretase and beta-secretase) which are to remove the entire extracellular domain in order to release membrane-anchored carboxy-terminal fragments which have been linked to the process of apoptosis. One of the most substantiated role for APP is in synaptic repair and formation, its epression is found to be elevated during neuronal differentiation and following any neural injury. Even though the native biological function of amyloid precursor protein is of particular interest to Alzheimer’s research, currently very little is known about this protein.

APP ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-APP antibody.
  • Standards: Concentrations 0, 0.938, 1.875, 3.75, 7.5, 15, 30, 60 ng/ml, made up from lyophilized recombinant human APP.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled APP Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of amyloid precursor protein (APP) using this human amyloid precursor protein ELISA kit was 0.563 ng/ml. The dynamic range for this assay is 0.938 – 60 ng/ml.

ASSAY CHARACTERISTICS

– Amyloid Precursor Protein (APP): ELISA
– Specificity: Highly specific for APP, no cross reactivity or interference between APP and analogues was detected.
– Recovery: Serum (89 – 101%), EDTA Plasma (87 – 100%), Heparin Plasma (87 – 105%).
– Linearity: Serum (85 – 105%), EDTA Plasma (83 – 95%), Heparin Plasma (84 – 89%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. The amyloid precursor protein and its network of interacting proteins: physiological and pathological implications. Brain Res Brain Res Rev. (2005) 48 (2): 257-64. Review. Russo C., et al.
  2. Insights into the physiological function of the β-amyloid precursor protein: beyond Alzheimer’s disease. J Neurochem. (2014) 129 (5): 756-69. Review. Dawkins E. and Small D.H.
  3. Amyloid precursor protein and neural development. Development. (2014) 141 (13): 2543-8. Review. Nicolas M. and Hassan B.A.
  4. Amyloid-β precursor protein: Multiple fragments, numerous transport routes and mechanisms. Exp Cell Res. (2015) 334 (1): 45-53. Review. Muresan V. and Ladescu Muresan Z.
  5. Roles of the amyloid precursor protein family in the peripheral nervous system. Mech Dev. (2013) 130 (6-8): 433-46. Review. Caldwell J.H., et al.

ADDITIONAL INFORMATION

  • Full Name: Amyloid Precursor Protein (APP) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Biological Fluids, Plasma, Tissue Homogenates
  • Sensitivity: 0.563 ng/ml

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