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Kidney Injury Molecule 1 (KIM-1) ELISA Kit

INTENDED USE

Human kidney injury molecule-1 ELISA kit can be used for detecting in vitro quantitative levels of kidney injury molecule 1 (KIM1) in human serum, plasma, biological fluids and tissue homogenates. This assay has a minimum detection limit of 0.94ng/ml.

BACKGROUND

Kidney injury molecule 1 (KIM-1, KIM1) is also called hepatitis A virus cellular receptor 1, it is a transmembrane glycoprotein which is 339 amino acid in length where the N-terminal ectodomain contains the mucin and immunoglobulin-like domains. KIM-1 normally exists in very low levels in normal kidneys, however when upregulated during injury, it is detectable in urine in a number of different human diseases. It has been identified as a potential biomarker for renal injury, acute kidney failure or acute tubular necrosis and it has a great importance in many kidney disorders and diseases.

Several new early biomarkers of acute kidney injury (AKI) have indicated KIM-1 among the most promising ones. KIM-1 has not only been discovered as a sensitive diagnostic marker but also a predictive value for AKI in patients who are undergoing cardiac surgery. Accordingly, it has also been recently used as a nephrotoxicity biomarker during preclinical studies of drug candidates and the Food and Drug Administration (FDA) to identify KIM-1 as an appropriate biomarker for renal injury in preclinical studies of pharmacologic agents.

KIM1 ELISA KIT CONTENT


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti KIM1 antibody.
  • Standards: Concentrations 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5.0, 10.0 ng/ml, made up from lyophilized recombinant human kidney injury molecule-1.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled KIM1 Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of kidney injury molecule 1 (KIM1) using this human kidney injury molecule-1 ELISA kit was 0.194 ng/ml. The dynamic range for this assay is 0.156 – 10 ng/ml.

ASSAY CHARACTERISTICS

– Kidney Injury Molecule-1 (KIM1): ELISA
– Specificity: Highly specific for KIM1, no cross reactivity or interference between KIM1 and analogues was detected.
– Recovery: Serum (86 – 102%), EDTA Plasma (86 – 104%), Heparin Plasma (88 – 105%).
– Linearity: Serum (88 – 105%), EDTA Plasma (86 – 98%), Heparin Plasma (87 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Molecular markers of kidney injury. Urol Oncol. (2013) 31 (5): 682-5. Review. Lane B.R.
  2. Kidney injury molecule-1 in kidney disease. Ren Fail. (2016) 38 (10): 1567-1573. Yin C. and Wang N.
  3. Urinary and serum biomarkers for the diagnosis of acute kidney injury: an in-depth review of the literature. Nephrol Dial Transplant. (2013) 28 (2): 254-73. Review. Vanmassenhove J., et al.
  4. Kidney injury molecule-1: more than just an injury marker of tubular epithelial cells? J Cell Physiol. (2013) 228 (5): 917-24. Review. Lim A.I., et al.
  5. Emerging biomarkers and metabolomics for assessing toxic nephropathy and acute kidney injury (AKI) in neonatology. Biomed Res Int. (2014) 2014: 602526. Review. Mussap M., et al.

ADDITIONAL INFORMATION

  • Full Name: Kidney Injury Molecule 1 (KIM-1) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Biological Fluids , Plasma, Tissue Homogenates
  • Sensitivity: 0.195 ng/ml

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