LH ELISA Kit (Luteinizing Hormone)

INTENDED USE

Human LH ELISA kit is a reliable quantitative procedure for measuring luteinizing hormone (LH) in human serum samples. This assay has a minimum sensitivity detection limit of 1.27 mIU/ml.

BACKGROUND

Luteinizing hormone (LH) has a molecular weight of 30,000 daltons and it consists of alpha and beta chains that are associated non-covalently. The α -subunit is 89 amino acid residues and the β-subunit consists of 129 amino acids, with the carbohydrate content being between 15%-30%. The hypothalamic-pituitary-gonadal axis controls fertility and measuring LH is crucial to determining this. Also, it can help the advent of in vitro fertilization (IVF) procedure in order to overcome infertility that is associated to various problems. Over the many year the vast improvement in methodology from bioassays that are technically very demanding to the many simple and rapid immunoassays have helped this cause considerably.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiterwells: Break apart strips which are coated with anti LH antibody (monoclonal).
• Standard 0-5: Lyophilized. Concentration: 0, 10, 20, 40, 100, 200 mIU/mL. The standards are calibrated against WHO 2nd International Standard for LH IRP (80/552).
• Enzyme Conjugate: Containing antibody conjugated to horseradish peroxidase.
• Substrate Solution: Tetramethylbenzidine (TMB).
• Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of luteinizing hormone (LH) using current LH ELISA kit was approximately 1.27 mIU/ml. The dynamic range for this assay is 10.0 – 200.0 mIU/ml.

ASSAY CHARACTERISTICS

– Assay Dynamic Range: 1.27 – 200 mIU/mL.
– Analytical Sensitivity: 1.27 mIU/ml
– Intra-Assay Variation: 4.57 – 7.62%
– Inter-Assay Variation: 4.45 – 11.02%
– Linearity: 88.0 – 105.5%
– Recovery: 89.7 – 107.4%
– Interfering Substances: None
– High-Dose-Hook Effect:  None detected up to 10,000 mIU/mL of LH.
– Drug Interferences: None

REFERENCES

1. Understanding the role of LH: myths and facts. Reprod Biomed Online. (2007) 15 (4): 468-77. Fischer R.
2. Benefits of luteinizing hormone activity in ovarian stimulation for IVF. Reprod Biomed Online. (2009) 18 Suppl 2: 31-6. Review. Venetis C.A., et al.
3. FSH and LH together in ovarian stimulation. Eur J Obstet Gynecol Reprod Biol. (2004) 115 Suppl 1: S34-9. Review. Levi-Setti P.E., et al.

ADDITIONAL INFORMATION

• Full Name: LH ELISA Kit (Luteinizing Hormone)
• Reactivity: Human
• Sample Type: Serum
• Sensitivity: 1.27 mIU/ml

OTHER RELATED ELISA KITS

• 17 OH Progesterone ELISA Kit
• FSH ELISA Kit
• DHEA ELISA Kit