Human luteinizing hormone ELISA kit is a reliable quantitative procedure for measuring luteinizing hormone (LH) in human serum samples. This assay has a minimum sensitivity detection limit of 1.27 mIU/ml.
Luteinizing hormone (LH) is a glycoprotein which is produced in both females and males in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH) from the anterior pituitary gland. It has a molecular weight of 30,000 daltons and consists of alpha and beta chains that are associated non-covalently. The α-subunit is identical to the other pituitary hormones namely chorionic gonadotropin (hCG), follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH), whilst the β-subunit is unique and confers the biological activity to the molecule. The α -subunit is made up of 89 amino acid residues and the β-subunit consists of 129 amino acids, with the carbohydrate content being between 15%-30%.
The importance of determining human luteinizing hormone (LH) is to ascertain the homeostasis of fertility regulation through the hypothalamic-pituitary-gonadal axis. Also, it can help the advent of in vitro fertilization (IVF) procedure in order to overcome infertility that is associated to various problems. Over the many year the vast improvement in methodology from bioassays that are technically very demanding to the many simple and rapid immunoassays have helped this cause considerably. In the differential diagnosis of pituitary, gonadal dysfunction or hypothalamic, a number of assays that measure the concentration of human LH have been routinely used in conjunction with human FSH due to the fact that their roles are inter-related. Other uses include pinpointing ovulation, determining menopause and monitoring endocrine therapy.
LUTEINIZING HORMONE ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiterwells: Break apart strips which are coated with anti LH antibody (monoclonal).
- Standard 0-5: Lyophilized anti human LH. Concentration: 0, 10, 20, 40, 100, 200 mIU/mL. The standards are calibrated against WHO 2nd International Standard for LH IRP (80/552).
- Enzyme Conjugate: Containing anti luteinizing hormone antibody conjugated to horseradish peroxidase.
- Substrate Solution: Tetramethylbenzidine (TMB).
- Stop Solution.
The minimum sensitivity detection limit of human luteinizing hormone (LH) using current luteinizing hormone ELISA kit was approximately 1.27 mIU/ml. The dynamic range for this assay is 10.0 – 200.0 mIU/ml.
– Luteinizing Hormone (LH): ELISA
– Assay Dynamic Range: 1.27 – 200 mIU/mL.
– Analytical Sensitivity: 1.27 mIU/ml
– Intra-Assay Variation: 4.57 – 7.62%
– Inter-Assay Variation: 4.45 – 11.02%
– Linearity: 88.0 – 105.5%
– Recovery: 89.7 – 107.4%
– Interfering Substances: None
– High-Dose-Hook Effect: None detected up to 10,000 mIU/mL of LH.
– Drug Interferences: None
- Understanding the role of LH: myths and facts. Reprod Biomed Online. (2007) 15 (4): 468-77. Fischer R.
- Benefits of luteinizing hormone activity in ovarian stimulation for IVF. Reprod Biomed Online. (2009) 18 Suppl 2: 31-6. Review. Venetis C.A., et al.
- FSH and LH together in ovarian stimulation. Eur J Obstet Gynecol Reprod Biol. (2004) 115 Suppl 1: S34-9. Review. Levi-Setti P.E., et al.
- Pituitary effects of steroid hormones on secretion of follicle-stimulating hormone and luteinizing hormone. Domest Anim Endocrinol. (2002) 23 (1-2): 33-42. Review. Nett T.M., et al.
- Full Name: Luteinizing Hormone ELISA Kit
- Reactivity: Human
- Sample Type: Serum
- Sensitivity: 1.27 mIU/ml