Bovine Leptospira hardjo ELISA Kit

Full Name: Bovine Leptospira hardjo ELISA Kit
Reactivity: Bovine
Sample Type: Serum, Milk
Sensitivity: Qualitative

BACKGROUND

Cattle are regarded as the predominant host reservoir for hardjo and there are two main types of pathogens of Leptospira hardjo for cattle, either interrogens serovar or pomona. The main source of infection arises from direct contact with contaminated urine or through products of abortion. Pomona is found to be more resistant to environmental conditions and also can be less host specific. In general, pomona is found to enter a herd through contaminated water or through infected cattle, whereas hardjo only enter as a result of infected cattle being introduced. Humans can become infected through direct touch with an infected animal, contact with contaminated water, or the lungs and kidneys. Penicillin and ceftriaxone are just two of the antimicrobials that “Leptospira hardjo” is sensitive to. The most typical symptom is acute renal failure, however it can also lead to pulmonary illness and cutaneous leishmaniasis (CL).

ELISA assay take advantage of using monoclonal antibodies that have been standardised in order to effectively catch and detect the specific antigen. The following procedure is designed to undertake a rapid screening test in order to specifically detecting Leptospira hardjo antibodies which may be present in either milk or serum samples of infected cattle.

INTENDED USE

Bovine Leptospira hardjo ELISA kit is designed for the detection of antibodies against vital polysaccharide epitope of Leptospira hardjo using samples of bovine serum and milk. This assay has a quantitative and qualitative standard range.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate: Coated with monoclonal antibodies.
• HRPO conjugated (100x Concentrated): Anti-bovine monoclonal antibody.
• Conjugate Diluent.
• Inactivated Positive Control (Freeze Dried).
• Inactivated Negative Control (Freeze Dried).
• Wash Solution (200x Concentrated).
• ELISA Buffer.
• Substrate Buffer A.
• Substrate Buffer B.
• Stop Solution.
• Plastic Cover Seal.

SENSITIVITY

The minimum sensitivity detection limit of antibodies against Leptospira hardjo polysaccharide epitope using current bovine Leptospira hardjo ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Mean absorption value of the negative control should be ≤ 0.200 OD units (450 nm).
– Mean absorption value of the positive control provided should be ≥ 0.800 OD units (450 nm).
– High prevalence is more than 15% positive animals.

REFERENCES

1. Evaluation of an ELISA for the diagnosis of experimentally induced and naturally occurring Leptospira hardjo infections in cattle. Vet Microbiol. (1990) 21 (3): 255-62. Bercovich Z., et al.
2. A review of laboratory techniques and their use in the diagnosis of Leptospira interrogans serovar hardjo infection in cattle. Aust Vet J. (1994) 71 (9): 290-4. Review. Smith C.R., et al.
3. Development of an ELISA to detect antibodies to a protective lipopolysaccharide fraction of Leptospira borgpetersenii serovar hardjo in cattle. Vet Microbiol. (1999) 69 (3): 173-87. Yan K.T., et al.
4. Leptospira_hardjo serodiagnosis: a comparison of MAT, ELISA and Immunocomb. Vet Rec. (1997) 141 (23): 603-4. Woodward M.J., et al.

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