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LEPR ELISA Kit (Leptin Receptor)

INTENDED USE

Human LEPR ELISA kit is a reliable quantitative procedure for measuring leptin receptor (LEPR, LEP-R) in human tissue homogenates, serum, biological fluids and plasma. This assay has a minimum sensitivity detection limit of < 9.375 pg/ml.

BACKGROUND

Leptin receptor (also called LEPR, LEP-R, obese receptor and OB-R) is a transmembrane protein that plays a role in regulating energy balance and body weight. It is expressed in many tissues including the hypothalamus, adipose tissue and other peripheral tissues. The main function is to bind to leptin, a hormone produced by adipose tissue and regulates food intake and energy expenditure through signalling in the hypothalamus.

LEPR is a G protein-coupled receptor that consists of 948 amino acids. It contains an extracellular domain that is responsible for binding to leptin, a transmembrane domain and an intracellular domain that is involved in signalling. The intracellular domain interacts with other signalling proteins to activate downstream pathways, leading to regulation of food intake and energy expenditure. Various LEP-R antagonists are being explored as a potential treatment for obesity due to their ability to regulate food intake and energy expenditure.

CONTENT

All reagents need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-LEPR antibody.
  • Standards: Concentrations 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled LEP-R Antibody. structu
  • Product Instructions.

SENSITIVITY

The minimum sensitivity detection limit of human leptin receptor (LEP-R, LEPR) using current LEPR ELISA kit was approximately < 8.0 pg/ml. The dynamic assay range for this assay is 156.0 – 10,000.0 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for LEPR, no cross reactivity or interference between LEP-R and analogues was detected.
– Recovery: Serum (89 – 104%), EDTA Plasma (87 – 104%), Heparin Plasma (86 – 104%).
– Linearity: Serum (85 – 102%), EDTA Plasma (82 – 100%), Heparin Plasma (82 – 99%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Hypertension in obesity and the leptin receptor gene locus. J. Clin. Endocr. Metab. (2000) 85: 3126-3131. Rosmond, R., et al.
  2. Identification and expression cloning of a leptin-receptor, OB-R. Cell (1995) 83: 1263-1271. Tartaglia, L. A., et al.
  3. Polymorphisms in the leptin receptor (LEPR)–putative association with obesity and T2DM. J. Hum. Genet. (2006) 51: 85-91. Park, K. S., et al.

ADDITIONAL INFORMATION

  • Full Name: LEPR ELISA Kit (Leptin Receptor)
  • Reactivity: Human
  • Sample Type: Plasma, Serum, Biological Fluids, Tissue Homogenates
  • Sensitivity: < 9.375 pg/ml

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