Leptin ELISA Kit

INTENDED USE

Human leptin ELISA kit is a reliable quantitative procedure for measuring leptin in human serum, plasma (EDTA, heparin) and cell culture supernatant. This assay has a minimum sensitivity detection limit of < 0.25 ng/ml.

BACKGROUND

Leptin which is the product of the obese (OB) gene is a single chain of 146 amino acids sequence that is 16kDa in size. It is a circulating hormone which can be found to be abundantly and more specifically expressed in the adipose tissues, playing a vital function in fat metabolism, body weight regulation and appetite control. It is released from fat cells that are located in the adipose tissue. It is able to send signals to the brain (especially the hypothalamus area).

Leptin has a dual action of either increasing energy consumption or decreasing the appetite for food, leading to a net effect of more fat being burnt. In addition to its role in metabolic control, there is evidence it is important in; neuroendocrine signalling, reproduction and is a powerful inhibitor of bone formation.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate: Coated with mouse anti leptin antibody. Wells are separately breakable. (8×12) wells.
• Standards A-E: Lyophilized recombinant, concentrations 1, 10, 25, 50, 100 ng/mL.
• Control 1 And Control 2.
• Antibody-HRP-Conjugate: Consisting of mouse antibody biotinylated and streptavidin horseradish peroxidase conjugate.
• Dilution Buffer.
• Washing Buffer (20x Concentrated).
• TMB Substrate.
• Stopping Solution: 0.2 M sulphuric acid.
• Sealing Tape: To cover microtiter plate.
• Instructions For Use.
• Quality Certificate

SENSITIVITY

The minimum sensitivity detection limit of leptin using current leptin ELISA kit was approximately < 0.25 ng/ml. The dynamic range for this assay is 1 – 100 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity: 1 ng/ml
– Intra-Assay Variance: 4.71 – 5.18%
– Inter-Assay Variance: 8.33 – 19.21%
– Linearity And Sample Dilution: 4.96 – 38.06%
– Recovery: 100 – 116% (5ug/L), 98 – 118% (10ug/L).
– Interference: None
– Species Cross Reactivity: No cross reaction with the following species: Mouse, Rat, Cat, Dog, Guinea Pig, Rabbit, Chicken, Sheep, Goat, Pig, Cow, Donkey, Horse.

REFERENCES

1. Serum Leptin-level is a regulator of bone mass. Proc. Nat. Acad. Sci. (2004) 101: 3258-3263. Elefteriou, F., et al.
2. Nonadipose tissue production of Leptin: Leptin as a novel placenta-derived hormone in humans. Nature Med. (1997) 3: 1029-1033. Masuzaki, H., et al.
3. The human obese (OB) gene: RNA expression pattern and mapping on the physical, cytogenetic, and genetic maps of chromosome 7. Genome Res. (1995) 5: 5-12. Green, E. D., et al.
4. Human obese gene expression: adipocyte-specific expression and regional differences in the adipose tissue. Diabetes (1995) 44: 855-858. Masuzaki, H., et al.

ADDITIONAL INFORMATION

• Full Name: Leptin ELISA Kit
• Reactivity: Human
• Sample Type: Serum, Plasma (Heparin, EDTA), Cell Supernatant
• Sensitivity: < 0.25 ng/ml

OTHER RELATED ELISA KITS

• Leptin Receptor ELISA Kit
• Insulin ELISA Kit
• Human Growth Hormone ELISA Kit