PMN Elastase ELISA Kit (Polymorphonuclear Elastase)

Full Name:  PMN Elastase ELISA Kit (Polymorphonuclear Elastase)
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 0.5 U/ml

BACKGROUND

PMN elastase is part of the subfamily serine protease that hydrolyse many proteins such as elastin, it is formed as an inactive zymogen (within the pancreas) and becomes active by trypsin (within the duodenum). Neutrophil elastases are one of the main protease enzymes, they tend to be 218 amino acids I length and contains two asparagine-linked carbohydrate chains that are important for glycosylation. It hydrolyses proteins within specialized neutrophil lysosomes (known as azurophil granules) or extracellular matrix proteins and when neutrophil elastase is expressed aberrantly there is evidence that it can cause emphysematous and emphysema changes. It mainly occurs in polymorph nuclear neutrophil granulocytes in endothelial and macrophages cells.

INTENDED USE

Human PMN elastase ELISA kit is designed for measuring in vitro quantitative levels of IgG auto-antibodies to polymorphonuclear elastase (PMN elastase) in human plasma and serum. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiterplate: Break apart strips, containing wells coated with polyclonal antibodies.
• Calibrator: Concentrations 15.6, 13.3, 62.5, 125, 250, 500, 1,000 ng/ml, made up from lyophilized.
• Controls 1 And Control 2.
• Enzyme Conjugate: Anti-alpha 1 PI antibody, containing polyclonal antibodies labelled with horseradish peroxidase.
• Calibrator/Sample Diluent.
• Substrate Solution: Tetramethylbenzidine (TMB).
• Stop Solution: Contains 2 M hydrochloric acid. Avoid contact with the stop solution. It may cause skin irritations and burns.
• Wash Solution (10x Concentrated).

SENSITIVITY

The minimum detection sensitivity level of IgG antibodies to human polymorphonuclear elastase (PMN) elastase or polymorph nuclear elastase using current PMN elastase ELISA kit was 0.5 U/ml. The dynamic range for this assay is 6.3 – 100.0 U/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity: 0.2 ng/ml.
– Specificity: Highly specific for human PMN elastase only, which also includes PMN elastase/alpha1-PI complex.
– Intra Assay Precision: 4.6 – 5.2%
– Inter Assay Precision: 3.6 – 5.4%
– Recovery: 96 – 110%
– Linearity: 90 – 120%
– Effects Of Hemolysis And Bilirubin: Samples displaying hemolysis and bilirubin (up to 200 mg/l), i.e. severe icterus display no clinical significant effect on this procedure.

REFERENCES

1. Platelets, Micro-Particles and Elastase. A Review with Extrapolation to the Mechanism of Generation and Bio-Pathology of Platelet Fragments. Pathol Oncol Res. (2017) 23 (2): 455-458. Robert L. and Labat-Robert J.
2. Interaction between elastin and elastases and its role in the aging of the arterial wall, skin and other connective tissues. A review. Mech Ageing Dev. (1984) 28 (2-3): 155-66. Review. Robert L., et al.
3. Elastase-induced pulmonary emphysema: insights from experimental models. An Acad Bras Cienc. (2011) 83 (4): 1385-96. Review. Antunes M.A. and Rocco P.R.
4. Anti-inflammatory activity of neutrophil elastase inhibitors. Curr Opin Investig Drugs. (2003) 4 (5): 556-65. Review. Tremblay G.M., et al.

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