Mouse IL-6 ELISA Kit (Interleukin 6)

Full Name: Mouse IL-6 ELISA Kit (Interleukin 6)
Reactivity: Mouse
Sample Type: Serum, Plasma, Cell Culture Media
Sensitivity: 1.07 pg/ml


Interleukin 6 (IL-6) is crucial for several biological processes. It controls immune responses, nerve cell activity, nerve cell regulation, hematopoiesis, acute phase reactions and host defence. Several of the cytokines in the IL-6 superfamily need gp130 to activate. Rat, mouse and human cDNAs have all been successfully cloned in an effort to better comprehend these cytokines. and their actions. IL -6 increases expression of the neurotransmitter norepinephrine in the brain, which may contribute to cardiovascular regulation, neural development and stress response. The primary IL-6 receptor is gp130, but other receptors have been identified.

Several distinct triggers, including antigenic or mitogenic activation, lipopolysaccharides, calcium ionophores, cytokines and viruses, cause different cells to produce IL-6. These signals initiate its emergence. In both their normal and transformed states, it is found in both lymphoid and non-lymphoid cells. It has been discovered that an excess of IL-6 in the serum may be a sign of a variety of conditions including; infections, autoimmune conditions, trauma and even cancer.


Mouse IL-6 ELISA kit is designed for measuring quantitative concentrations of interleukin 6 (IL6, IL-6) using mouse plasma, serum and cell culture media. This assay has a minimum sensitivity detection limit of 1.07 pg/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Pre-Coated Plate: Anti-mouse IL-6 rabbit IgG.
  • Labelled Antibody (30x Conc.).
  • Standard.
  • EIA Buffer.
  • Solution For Labelled Antibody.
  • Chromogen (TMB).
  • Stop Solution.
  • Wash Buffer (40x Conc.).


The standard range of interleukin 6 (IL6) using current mouse IL-6 ELISA kit was approximately 1.07 pg/ml. The dynamic range for this assay is 10.94 – 700 pg/ml.


– Titer Assay: 57.6 – 103.6%
– Added Recovery Assay: 79.2 – 106.2%
– Intra – Assay: 3.5 – 6.8%
– Inter – Assay: 3.6 – 6.2%
– Specificity: Mouse IL-6 (100.0%), Mouse Leptin (≦0.1%), Mouse MIP-2 (≦0.1%), Mouse OPN (≦0.1%).
– Sensitivity: 1.07 pg/mL


  1. IL-6/Stat3-driven pulmonary inflammation, but not emphysema, is dependent on interleukin-17A in mice. Respirology. (2014) 19 (3): 419-27. Ruwanpura S.M., et al.
  2. A critical role of the transcription factor fli-1 in murine lupus development by regulation of interleukin-6 expression. Arthritis Rheumatol. (2014) 66 (12): 3436-44. Sato S., et al.
  3. Targeting_IL-6 by both passive or active immunization strategies prevents bleomycin-induced skin fibrosis. Arthritis Res Ther. (2014) 16 (4): R157. Desallais L., et al.
  4. Obesity and IL-6-interact in modulating the response to endotoxemia in mice. Cytokine. (2013) 61 (1): 71-7. Pini M., et al.


Online Enquiry Form

"*" indicates required fields

Please check mark information required:


Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford