Insulin ELISA Kit (Rat)


Rat insulin ELISA kit can be used for quantitative analysis of insulin levels using rat serum or plasma. This assay has a minimum sensitivity detection limit of 0.01 ng/ml.


Insulin is a peptide hormone which is produced and secreted by the beta cells found in the pancreatic islets. It is important for regulating metabolism of carbohydrates, proteins and fats by promoting the absorption of glucose from the blood into liver, skeletal muscle and fat cells. This glucose is able to serve as a form of energy to various cells or it be converted into fat so that it can be used at a later point. Insulin is also known to affect many other metabolic processes for example the breakdown of proteins and fats. Diabetes is one of the most common problem that has been linked to insulin, this is known to occur in individuals where the body is unable to secrete enough insulin or when the body is unable to utilise the secreted insulin effectively.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Coated Plate: Mouse monoclonal anti-insulin.
  • Calibrators (1-5): Concentration ranging between 0.156 – 10 ng/ml.
  • Calibrator 0.
  • Enzyme Conjugate.
  • Enzyme Conjugate Buffer.
  • Wash Buffer.
  • Substrate TMB.
  • Stop Solution.


The standard range of insulin using current rat insulin ELISA kit was approximately 0.01 ng/ml. The dynamic range for this assay is 0.156 – 10 ng/ml.


– Rat Insulin: ELISA
– Detection limit: ≤0.15 μg/l
– Hook effect: Concentration up to at least 450 μg/l can be measured without giving falsely low results.
– Intra-assay-Variation: 3.2 – 10.0%
– Inter-assay-Variation: 2.8 – 5.1%
– Specificity: Human insulin (167%), Human proinsulin (75%), Human C-peptide (< 0.05%), Insulin lispro (Humalog® ) (167%), IGF-I (< 0.02%), IGF-II (< 0.02%), Rat C-peptide (< 0.001%), Rat proinsulin (7%), Mouse insulin (75%), Porcine Insulin (476%), Sheep Insulin (179%), Bovine Insulin (78%).


  1. A quantitative enzyme-linked immunosorbent assay for rat insulin. J Immunoassay. (1988) 9 (2): 135-58. Bank H.L.
  2. Insulin therapy normalizes reduced myocardial β-adrenoceptors at both the onset and after sustained hyperglycemia in diabetic rats. Life Sci. (2015) 132: 101-7. Haley J.M., et al.
  3. Development of an ultrasensitive immunoassay using affinity maturated antibodies for the measurement of rodent insulin. Anal Biochem. (2015) 473: 72-9. Imai S., et al.
  4. Long-term treatment with atrial natriuretic peptide inhibits ATP production and insulin secretion in rat pancreatic islets. Am J Physiol Endocrinol Metab. (2011) 300 (3): E435-44. You H. and Laychock S.G.


  • Full Name: Insulin ELISA Kit (Rat)
  • Reactivity: Rat
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.01 ng/ml



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PhD Student / University of Glasgow

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Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

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Senior Technician / Addenbrooke’s Hospital

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