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Interleukin-12 P70 (IL-12 P70) ELISA Kit

INTENDED USE

Human IL-12 p70 ELISA kit can be used to analyse in vitro quantitative levels of interleukin-12 p70 (IL-12 p70) in human serum, tissue homogenates, plasma and other biological fluids. This assay has a minimum detection limit of 4.6 pg/ml.

BACKGROUND

Interleukin-12 (IL-12) is a pleiotropic cytokine which also use to be called natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF), is primarily produced by stimulated macrophages. It is a 70-KDa cytokine that is composed of two disulfide-linked proteins (p35 and p40), IL-12p70 is a heterodimer of p35 and p40 subunits and it is a vital cytokine that is secreted by antigen-presenting cells in response to antigenic stimulation.

Gene expression studies have indicated that both strains of the IL-12 cytokine family can induce high levels of p40 (protein chain that is communal to both IL-12 p70 and IL-23) and p19 (a subunit of IL-23). Conversely it was found that only infection with ACT-18HS19 were able to consistently induced the transcription of IL-12 p35 (a subunit of IL-12 p70).

Interleukin-12 was originally discovered as factor being produced by human Epstein-Barr-Virus transformed B cell lines. It was also shown to be a pro-inflammatory cytokine that can be produced by B cells, phagocytic cells and many antigen-presenting cells that have the ability to modulate the adaptive immune responses by generating T-helper type 1 cells. IL-12 can exert a number of different biological effects on natural killer cells and human T cells.

There is a strong suggestion of a potential usefulness in cancer therapy, due to its ability to direct the development of a Th1 type immune response from naive T cells indicating a critical role in regulating an immune response. Increased plasma levels of IL-12 have been found in neurological disorders and a significant elevations have been measured in patients with multiple sclerosis, autistic, rheumatoid arthritis, seronegative spondylarthropathies and osteoarthritis.

IL-12 P70 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human IL-12 p70 antibody.
  • Standards: Concentrations 0, 7.81, 15.625, 31.3, 62.5, 125, 250, 500 pg/ml, made up from lyophilized recombinant human interleukin-12 p70.
  • Sample/Standard Dilution Buffer.
  • Assay Dilution.
  • Biotinylated-labelled IL-12 p40 Antibody.
  • HRP-Streptavidin (HRP-SA).
  • Biotin System (BS).
  • BS Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interleukin-12 p70 (IL-12 p70) using this human IL-12 p70 ELISA kit was approximately 4.6 pg/ml. The dynamic range for this assay is 7.8 – 500 pg/ml.

ASSAY CHARACTERISTICS

– Interleukin-12 P70 (IL-12 P70): ELISA
– Specificity: Highly specific for IL-12 p70, no cross reactivity or interference between IL-12 p70 and analogues was detected.
– Recovery: Serum (86 – 100%), EDTA Plasma (87 – 102%), Heparin Plasma (86 – 105%).
– Linearity: Serum (86 – 103%), EDTA Plasma (83 – 99%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Genetic variations in interleukin-12 related genes in immune-mediated diseases. J Autoimmun. (2012) 39 (4): 359-68. Review. van Wanrooij R.L., et al.
  2. Stepwise regulation of TH1 responses in autoimmunity: IL-12-related cytokines and their receptors. Inflamm Bowel Dis. (2005) 11 (8): 755-64. Review. Becker C., et al.
  3. Novel IL-12 family members shed light on the orchestration of Th1 responses. Trends Immunol. (2003) 24 (4): 207-12. Review. Brombacher F., et al
  4. IL-12: the role of p4 0versus p75. Scand J Immunol. (2002) 56 (1): 1-11. Review. Abdi K.
  5. Interleukin-12: Functional activities and implications for disease. Cytokine Growth Factor Rev. (2015) 26 (5): 559-68. Review. Zundler S. and Neurath M.F.
  6. Inhibiting cytokines of the interleukin-12 family: recent advances and novel challenges. J Pharm Pharmacol. (2004) 56 (2): 145-60. Review. Vandenbroeck K., et al.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-12 P70 (IL-12 P70) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Biological Fluids, Plasma, Tissue Homogenates
  • Sensitivity: 4.6 pg/ml

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