IL-12 P70 ELISA Kit (Interleukin-12 p70)

INTENDED USE

Human IL-12 p70 ELISA kit can be used to analyse in vitro quantitative levels of interleukin-12 p70 (IL-12 p70) in human serum, tissue homogenates, plasma and other biological fluids. This assay has a minimum detection limit of 4.6 pg/ml.

BACKGROUND

Interleukin-12 (IL-12) is also called natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF). It was originally discovered as factor being produced by human Epstein-Barr-Virus transformed B cell lines. It is a 70-KDa protein in size and is composed of two disulfide-linked proteins (p35 and p40). It is a heterodimer that is joined by that is secreted by antigen-presenting cells in response to antigenic stimulation. It can exert a number of different biological effects on natural killer cells and human T cells.

There is a strong suggestion of a potential usefulness of IL-12 in cancer therapy, due to its ability to direct the development of a Th1 type immune response from naive T cells indicating a critical role in regulating an immune response.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-human IL-12 p70 antibody.
• Standards: Concentrations 0, 7.81, 15.625, 31.3, 62.5, 125, 250, 500 pg/ml.
• Sample/Standard Dilution Buffer.
• Assay Dilution.
• Biotinylated-Labelled-Antibody.
• HRP-Streptavidin (HRP-SA).
• Biotin System (BS).
• BS Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interleukin-12 p70 (IL-12 p70) using this IL-12 p70 ELISA kit was approximately 4.6 pg/ml. The dynamic range for this assay is 7.8 – 500 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-12 p70, no cross reactivity or interference between IL-12 p70 and analogues was detected.
– Recovery: Serum (86 – 100%), EDTA Plasma (87 – 102%), Heparin Plasma (86 – 105%).
– Linearity: Serum (86 – 103%), EDTA Plasma (83 – 99%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Genetic variations in interleukin-12 related genes in immune-mediated diseases. J Autoimmun. (2012) 39 (4): 359-68. Review. van Wanrooij R.L., et al.
2. Stepwise regulation of TH1 responses in autoimmunity: IL-12-related cytokines and their receptors. Inflamm Bowel Dis. (2005) 11 (8): 755-64. Review. Becker C., et al.
3. IL-12: the role of p4 0versus p75. Scand J Immunol. (2002) 56 (1): 1-11. Review. Abdi K.
4. Interleukin-12: Functional activities and implications for disease. Cytokine Growth Factor Rev. (2015) 26 (5): 559-68. Review. Zundler S. and Neurath M.F.

ADDITIONAL INFORMATION

• Full Name: IL-12 P70 ELISA Kit (Interleukin-12 p70)
• Reactivity: Human
• Sample Type: Serum, Biological Fluids, Plasma, Tissue Homogenates
• Sensitivity: 4.6 pg/ml

OTHER RELATED ELISA KITS

• IL-12 P40 ELISA Kit
• IL-4 ELISA Kit
• IL-2 ELISA Kit