IFN-Alpha ELISA Kit (Interferon-Alpha)

INTENDED USE

Human IFN-alpha ELISA kit is designed for analysing in vitro quantitative concentrations of interferon-alpha (IFN-a, IFN-alpha, IFN-α) in human plasma, serum, tissue homogenates and other biological fluids. This assay has a minimum detection limit of 9.375 pg/ml.

BACKGROUND

Interferon-alpha (IFN-alpha or IFN-a) main function is modulate the response of the immune system to cancer, viruses, bacteria and other foreign substances that invade the body. There have been more than twenty distinct IFNs that have been identified in humans and animals. There are three main classes that have been identified alpha, beta and gamma.

IFN-alpha do not directly kill cancerous or viral cells but instead they are able to boost the immune system response in order to reduce the growth of cancer cells through regulating the action of several genes that can affect growth. Interferon are typically divided between three classes Type I, II and III IFNs. IFN-alpha is predominately produced by lymphoblastic cells, macrophages or monocytes and fibroblasts, however many other virus activated cells also have the ability to produce this cytokine. IFNα is able to bind to specific transmembrane interferon receptors, this can lead to the activation of various signalling pathways such as MAP kinase, JAK-STAT, IRS signalling and Crk-pathway.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-human IFN-alpha antibody.
• Standards: Concentrations 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml, made up from lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-Labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interferon-alpha (IFN-a, IFN-α, IFN-alpha) using current IFN-alpha ELISA kit was 9.375 pg/ml. The dynamic range for this assay is 15.6 – 1,000 ng/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IFN alpha, no cross reactivity or interference between IFN alpha and analogues was detected.
– Recovery: Serum (87 – 98%), EDTA Plasma (89 – 99%), Heparin Plasma (87 – 100%).
– Linearity: Serum (88 – 94%), EDTA Plasma (85 – 100%), Heparin Plasma (81 – 96%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Interferon at the crossroads of allergy and viral infections. J Leukoc Biol. (2015) 98 (2): 185-94. Review. Gonzales-van Horn S.R. and Farrar J.D.
2. Recent advances on the immunomodulatory effects of IFN-alpha: implications for cancer immunotherapy and autoimmunity. Autoimmunity. (2010) 43 (3): 204-9. Review. Rizza P., et al.
3. IFN-α subtypes: distinct biological activities in anti-viral therapy. Br J Pharmacol. (2013) 168 (5): 1048-58. Review. Gibbert K., et al.
4. New pharmacokinetic and pharmacodynamic tools for interferon-alpha (IFN-alpha) treatment of human cancer. Cancer Immunol Immunother. (2005) 54 (1): 1-10. Review. Tagliaferri P., et al.
5. New aspects of IFN-alpha/beta signalling in immunity, oncogenesis and bone metabolism. Cancer Sci. (2003) 94 (5): 405-11. Review. Takaoka A. and Taniguchi T.
6. Therapeutic alpha-interferons protein: structure, production, and biosimilar. Prep Biochem Biotechnol. (2015) 45 (2): 109-27. Review. El-Baky N.A. and Redwan E.M.

ADDITIONAL INFORMATION

• Full Name: IFN-Alpha ELISA Kit (Interferon-Alpha)
• Reactivity: Human
• Sample Type: Biological Fluids, Serum, Tissue Homogenates, Plasma
• Sensitivity: 9.375 pg/ml

OTHER RELATED ELISA KITS

• IFN-Gamma ELISA Kit
• TNF-Alpha ELISA Kit
• Insulin ELISA Kit