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Inhibin A ELISA Kit

INTENDED USE

Human inhibin A ELISA kit is a reliable quantitative procedure for measuring dimeric inhibin A in human serum and plasma samples. This assay has a minimum sensitivity detection limit of 1.0 pg/ml.

BACKGROUND

Inhibin A is found to be the predominant form of inhibin within the maternal circulation after 4 weeks of gestation. There have also many applications for determining levels during the early stages of pregnancy such as preeclampsia, miscarriage, fetal growth restriction and Down’s syndrome (Trisomy 21). It has also been used as a suitable markers for epithelial tumors of the mucinous type where over 20% of cases having displayed elevated levels.

Inhibin A has also been reported that approx. 70% of granulosa cell tumors show elevated levels too, the frequency of these elevations seems to vary among different studies which is likely due to difference in specificities of the antibodies utilised for analysis. However, it has been found to have a limited role as an ovarian cancer marker during pre-menopausal women because the circulating levels are higher and tend to fluctuate throughout the menstrual cycle.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiterwells 12 x 8 (break apart) strips: Wells coated with anti human inhibin A antibody (monoclonal).
  • Standards 1-5: Lyophilized human inhibin A, concentration 9.5 – 1,000.0 pg/ml.
  • Sample Diluent.
  • Control 1 And Control 2.
  • Enzyme Conjugate: Consisting of antibody conjugated to horseradish peroxidase.
  • Substrate Solution: Tetramethylbenzidine (TMB).
  • Stop Solution: Contains 0.5 M H2SO4, Avoid contact with the stop solution. It may cause skin irritations and burns.

SENSITIVITY

The minimum sensitivity detection limit of inhibin A using current inhibin A ELISA kit was approximately 1.0 pg/ml. The dynamic range for this assay is 9.5 – 1,000.0 pg/ml.

REFERENCES

  1. Inhibins as diagnostic markers in human reproduction. Adv Clin Chem. (2008) 45: 1-29. Review. Tsigkou A., et al.
  2. Clinical application of inhibin a measurement: prenatal serum screening for Down syndrome. Semin Reprod Med. (2004) 22 (3): 235-42. Review. Lambert-Messerlian G.M. and Canick J.A.
  3. Measurement of inhibin A and activin A in pregnancy–possible diagnostic applications. Mol Cell Endocrinol. (2001) 180 (1-2): 117-21. Review. Ledger WL.
  4. A summary analysis of Down syndrome markers in the late first trimester. Adv Clin Chem. (2007) 43: 177-210. Review. Palomaki G.E., et al.

ADDITIONAL INFORMATION

  • Full Name: Inhibin A ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.0 pg/ml

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