Activin A ELISA Kit

INTENDED USE

Human activin A ELISA kit is a reliable quantitative procedure for measuring bone activin A in human serum, cell culture supernatant, tissue lysates and body fluids. This assay has a minimum sensitivity detection limit of 37.5 pg/ml.

BACKGROUND

Activin A is member of the transforming growth factor-beta super family. It is predominately locally expressed by mesenchymal component in hemopoietic micro-environment. The expression can be regulated by a number of different cytokines at the mRNA level, in addition there are also several inhibitory molecules which are involved in tightly controlling the biological activity of this protein.

Activin A is a homodimeric glycoprotein which is synthesized by the decidua, placenta and fetal membranes. This plays a role in different biological processes such as; cell differentiation, proliferation and remodelling as well as morphogenesis. The main biological mechanism is its ability to bind and activate the type II receptor activin receptor-like kinase (ALK5) which, in turn, activates SMAD proteins.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Standard: Lyophilized human activin A standards. Concentrations 0, 62.5, 125, 250, 500, 1000, 2000, 4000.0 pg/ml.
• Microplate: Pre-coated with anti-human activin A antibody.
• Sample/Standard Diluent Buffer.
• Biotinylated Labelled Antobody (Concentrated).
• Antibody Diluent Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Stop Solution.
• Wash Buffer (25x Conc.).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum sensitivity detection limit of activin A using current activin A ELISA kit was approximately 37.5 pg/ml. The dynamic assay range for this assay is 62.5 – 4000.0 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for detecting activing A, displaying no cross reactivity or interference between other analogues.
– Recovery: Serum (89 – 105%), EDTA Plasma (88 – 102%), Heparin Plasma (91 – 103%).
– Linearity: Serum (90 – 104%), EDTA Plasma (82 – 99%), Heparin Plasma (82 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: The rate of loss is less than 10% within the expiration date, providing the kit is stored under appropriate conditions.

REFERENCES

1. Role of activin A and myostatin and their signaling pathway in human myometrial and leiomyoma cell function. J Clin Endocrinol Metab. (2014) 99 (5): E775-85. Islam M.S., et al.
2. Apoptosis modulation by activin-A and follistatin in human endometrial stromal cells. Gynecol Endocrinol. (2016) 32 (2): 161-5. Coutinho L.M., et al.
3. Human mammary gland and breast carcinoma contain immunoreactive inhibin/activin subunits: evidence for a secretion into cystic fluid. Eur J Endocrinol (1999) 141 (2): 190-4. Di, Loreto, C., et al.

ADDITIONAL INFORMATION

• Full Name: Activin A ELISA Kit
• Reactivity: Human
• Sample Type: Serum, Tissue Lysate, Cell Culture Supernatant, Body Fluids
• Sensitivity: 37.5 pg/ml

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