Humanin ELISA Kit (MT-RNR2)

INTENDED USE

Human Humanin ELISA kit can measure concentrations of MT-RNR2 (humanin) present is plasma, serum, biological fluids or tissue homogenate samples.This assay has a minimum sensitivity detection limit of 0.12 pg/ml.

INTRODUCTION

First identified in 2001, humanin is encoded in the 16S rRNA gene within the mitochondrial genome. At only 24 amino acids in length, it is the smallest known peptide encoded by the human mitochondria. Despite its small size, it has significant cytoprotective effects that researchers are only beginning to understand.

The most striking evidence relates to Alzheimer’s disease – levels in the brain decline with the progression of Alzheimer’s, and administering exogenous humanin appears to rescue neurons and improve memory in animal models. This neuroprotective effect arises from it’s ability to interact with the amyloid precursor protein, thereby reducing amyloid beta toxicity. Humanin may confer similar protection in diabetes by interacting with the insulin receptor to trigger downstream signaling cascades.

Excitingly, pharmaceutical companies have already developed synthetic-humanin analogs that show enhanced potency and bioavailability compared to the natural peptide. Early clinical trials demonstrate these humanin-analogs are safe, well-tolerated, and achieve much higher circulating levels then native when administered. Larger scale phase 2 and 3 efficacy trials will follow to determine if humanin-therapy can prevent neuronal deterioration in early Alzheimer’s disease or help regulate glucose metabolism in diabetes. If successful, analogs may soon provide clinicians powerful new tools to combat these age-related diseases.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-humanin.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this humanin ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.146, 0.586, 2.344, 9.375, 37.5, 150, 600 pg/ml
Reactivity: Human
Sensitivity: 0.12 pg/ml
Range: 0.146 – 600 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for humanin, no cross reactivity or interference between humanin and analogues was detected.
– Recovery: Serum (87 – 102%), EDTA Plasma (93 – 99%), Heparin Plasma (86 – 105%).
– Linearity: Serum (90 – 105%), EDTA Plasma (89 – 101%), Heparin Plasma (87 – 95%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Mitochondria-derived peptides in healthy ageing and therapy of age-related diseases. Adv Protein Chem Struct Biol. (2023) 136: 197-215. Dabravolski S.A.
2. Humanin: a mitochondrial signaling peptide as a biomarker for impaired fasting glucose-related oxidative stress. Physiol Rep. (2016) ;4 (9): e12796. Voigt A. and Jelinek H.F.

ADDITIONAL INFORMATION

• Full Name: Humanin ELISA Kit (MT-RNR2)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 0.12 pg/ml

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