HMGB1 ELISA Kit (High Mobility Group Protein B1)

Full Name: HMGB1 ELISA Kit (High Mobility Group Protein B1)
Reactivity: Human
Sample Type: Serum, Tissue Homogenates, Plasma, Biological Fluids,
Sensitivity: 18.75 pg/ml

INTRODUCTION

High mobility group protein B1 (HMGB1) is described as a non-histone chromatin-linked protein that functions in stabilising nucleosomes and thereby regulating transcription. It is a highly conserved protein that is displays approx. 99% homology between humans and rodents, it is abundant within the nucleus and expressed ubiquitously. It is a member of the hsp70/hsp90 family and it is an integral part of the heat-shock protein (HSP) response. HMGB1 can be released from cells by necrosis, stress (heat, ultraviolet light and ionizing radiation), apoptosis and cell death.

When released in response to tissue injury or other factors, HMGB1 accumulates under the surface of the plasma membrane to create a soluble pro-inflammatory state. The gene encoding human HMGB1 has been identified as SCR2 and is located on chromosome 6. It is essentially a nuclear protein that predominately binds to nucleosomes in promoting bending of DNA. It has the ability to bind damaged and distorted DNA, this allows it to undertake a wide array of functions such as DNA repair, cytosolic DNA recognition and chromatin structure regulation.

There are many cancer cells that display up-regulation of HMGB1 expression, the metastatic potential of many tumor cells was shown to show a direct relationship with the expression. This could be due its ability in modulating the adhesion cells, angiogenesis and extracellular matrix. The protein is able to catalyze this reaction, which uses oxygen radicals as an electron source during catalysis. The other important role that it performs is the activation of neutrophils, which moves oxygen and hydrogen peroxide to any site of infection.

INTENDED USE

Human HMGB1 ELISA kit can determine concentrations of high mobility group protein B1 (HMGB1, HMG-1, amphoterin, high mobility group box 1, non-histone chromosomal protein-1) using human samples of plasma, other biological samples, serum and tissue homogenates.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

One 96-Well Plate: Pre-coated with anti-HMGB1 antibody.
Standards: Lyophilized recombinant.
Sample/Standard Dilution Buffer.
Biotinylated-labelled Antibody.
Antibody Dilution Buffer.
HRP-Streptavidin Conjugate (SABC).
SABC Dilution Buffer.
TMB Substrate.
Wash Buffer (25x).
Plate Sealer.
Product Instructions.

TYPICAL RESULTS

For the following HMGB1 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml.
Reactivity: Human
Sensitivity: 18.75 pg/ml
Range: 31.25 – 2000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for HMGB1, no cross reactivity or interference between HMGB1 and analogues was detected.
– Recovery: Serum (85 – 101%), EDTA Plasma (95 – 105%), Heparin Plasma (91 – 103%).
– Linearity: Serum (83 – 97%), EDTA Plasma (86 – 99%), Heparin Plasma (81 – 96%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

Analysis of Circulating HMGB1 in Human Serum. Methods Mol Biol. (2020) 2108: 15-28. Chen W., et al.
HMGB1-containing nucleosome mediates chemotherapy-induced metastasis of human lung cancer. Biochem Biophys Res Commun. (2018) 500 (3): 758-764. Wang K., et al.
High Mobility Group Box-1 (HMGB1) Induces Toll-Like Receptor 4-Mediated Production of the Immunosuppressive Protein Galectin-9 in Human Cancer Cells. Front Immunol. (2021) 12: 675731. Teo Hansen Selnø A., et al.
HMGB1-activated fibroblasts promote breast cancer cells metastasis via RAGE/aerobic glycolysis. Neoplasma. (2021) 68 (1): 71-78. Chen Y., et al.

OTHER RELATED ELISA KITS

Online Enquiry Form

"*" indicates required fields