Histamine Cell Culture ELISA Kit
INTENDED USE
Human histamine cell culture ELISA kit is a method for detecting in vitro quantitative concentrations of histamine (beta imidazolethylamine) in human cell culture, urine and whole blood samples. This assay has a minimum detection level of 0.2 ng/ml.
BACKGROUND
Histamine (beta imidazolethylamine) is stored mostly in the form of granules of tissue mast cells (also found in basophils), is present in all tissues in the human body. It can be classified as an amine molecule due to the structure of the ammonia and it is predominately formed by the removal of a carboxyl group from the amino acid histidine.
Histamine can carry out its functions via binding to specific histamine receptors that are found on the surface of cells. Currently, there have been four receptors that have been identified namely H1, H2, H3 and H4. Histamine is associated with a number of vital functions such as stimulating gastric acid secretion in the stomach, increasing the heart rate, helping with contraction of smooth muscles and increasing permeability and lowering blood pressure. Its activity can also be blocked by some specific drugs which are referred to as anti-histamines. These are able to prevent histamine from binding to its receptor thereby preventing them from performing a particular function. The use of anti histamine is useful in the treatment of allergies and also useful in helping to heal peptic ulcers.
HISTAMINE CELL CULTURE ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Reaction Plate: 1x 96 well plate, empty.
- Wash Buffer Concentrate (50x).
- Diluent.
- Substrate (TMB).
- Stop Solution.
- Histamine Microtiter Strips: Antigen pre-coated microwell plate.
- Standards (A-F): Concentrations 0, 0.5, 1.5, 5, 15, 50 ng/ml.
- Control 1.
- Control 2.
- Antiserum: Goat anti-histamine antibody.
- Acylation Buffer.
- Acylation Reagent.
- Enzyme Conjugate.
SENSITIVITY
The minimum sensitivity limit of beta imidazolethylamine (histamine) using current histamine cell culture ELISA kit was approximately 0.2 ng/ml. The dynamic range for this assay is 0.5 – 50 ng/ml.
ASSAY CHARACTERISTICS
– Histamine (Cell Culture): ELISA
– Analytical Sensitivity (Limit of Detection): 0.2 ng/mL
– Analytical Specificity (Cross Reactivity): Hiatamine (100%), 3-Methyl-Histamine (0.1%), Tyramine (0.01%), L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin (< 0.001%).
– Intra Assay Precision: 5.6 – 8.0%
– Inter Assay Precision: 6.3 – 12.0%
– Linearity: 85.0 – 106.0%
– Recovery: 92 – 120%
REFERENCES
- Optogenetic probing of fast glutamatergic transmission from hypocretin/orexin to histamine neurons in situ. J Neurosci. (2012) 32 (36): 12437-43. Schöne C., et al.
- Histamine production by human neutrophils. FASEB J. (2013) 27 (7): 2902-10. Alcañiz L., et al.
- Exploring polyamine regulation by nascent histamine in a human-transfected cell model. Amino Acids. (2010) 38 (2): 561-73. Abrighach H., et al.
- Histamine prevents apoptosis in human monocytes. Clin Exp Allergy. (2007) 37 (3): 323-30. Soga F., et al.
- Effect of histamine H1 receptor antagonists on TARC/CCL17 and MDC/CCL22 production from CD14+ cells induced by antigenic stimulation in vitro. Int Arch Allergy Immunol. (2011) 155 (1): 38-51. Shoji N., et al.
ADDITIONAL INFORMATION
- Full Name: Histamine Cell Culture ELISA Kit
- Reactivity: Human
- Sample Type: Cell Culture, Whole Blood, Urine
- Sensitivity: 0.2 ng/ml
