HSV-2 IgG ELISA Kit (Herpes Simplex Virus 2)

Full Name: HSV-2 IgG ELISA Kit (Herpes Simplex Virus 2)
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 0.98 U/ml


Herpes simplex virus type 2 (HSV-2) is a genital herpes which is transmitted either through sexual activity or by any skin-on-skin contact with an open sore. It can spread through the secretions from the mouth or the genitals and it is highly contagious.

Blood tests are carried out to diagnose infections, antibodies are identified against HSV-1 and HSV-2. However, the “gold standard” for diagnosis of HSV infection remains to be isolation of the virus from tissue culture.


Human HSV-2 IgG ELISA kit is intended for detecting in-vitro amounts of human IgG antibodies against herpes simplex virus 2 (HSV-2 IgG) in samples of serum or plasma. This assay has a minimum analytical sensitivity limit of 0.98 U/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Herpes Virus 2 antigen coated microtiter strips.
  • Calibrator B (Cut-Off Standard).
  • Calibrator A (Negative Control).
  • Calibrator C (Weak Positive Control).
  • Calibrator D (Positive Control).
  • Sample Diluent.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Washing Buffer (10x).
  • Stop Solution.


The minimum detection sensitivity level of human IgG antibodies to herpes simplex virus-2 (HSV-2 IgG) using current HSV-2 IgG ELISA kit was 0.98 U/ml. The dynamic range for this assay is 1.0 – 150.0 U/ml.


– Intra-Assay-Precision: 9.3 %
– Inter-Assay-Precision: 7.6 %
– Inter-Lot-Precision: 3.3 – 8.8 %
– Analytical Sensitivity: 0.98 U/mL
– Recovery: 97 – 114 %
– Linearity: 81 – 107 %
– Cross-Reactivity: No cross-reactivity to Mumps, Varicella and Measles.
– Interferences: No interferences to hemoglobin up to 8.0 mg/mL, triglycerides up to 5.0 mg/mL and bilirubin up to 0.3 mg/mL.
– Clinical Specificity: 100 %
– Clinical Sensitivity: 100 %


  1. Peptide based enzyme-linked immunoassays for detection of anti-HSV-2 IgG in human sera. J Virol Methods. (2000) 87 (1-2): 63-70. Oladepo D.K., et al.
  2. An ELISA technique to detect IgG antibody to the early herpes simplex virus type 2 (HSV-2) antigen AG-4 in HSV-2 patients. J Virol Methods. (1983) 6 (5): 245-54. Evans L., et al.
  3. Prevalence and incidence estimation of HSV-2 by two IgG ELISA methods among South African women at high risk of HIV. PLoS One. (2015) 10 (3): e0120207. De Baetselier I., et al.
  4. Serological Profile of HSV-2 in STD Patients: Evaluation of Diagnostic Utility of HSV-2 IgM and IgG Detection. J Clin Diagn Res. (2014) 8 (12): DC16-9. Amudha V.P., et al.


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