GABA ELISA Kit (Gamma-Aminobutyric Acid)

INTENDED USE

Human GABA ELISA kit is designed for determining in vitro quantitative concentrations of gamma aminobutyric acid (GABA) in human serum, plasma, biological fluids and tissue homogenates. This assay has a minimum sensitivity detection limit of 18.75 pg/ml.

BACKGROUND

Gamma aminobutyric acid (GABA) is an inhibitory neurotransmitter, which functions to slow down or weaken neuron signals. It occurs naturally within the brain where it is vital in regulating the brain activity, there is also evidence to supports its important role in regulating muscle tone. Very small amounts are obtained from food, however, the majority of it is synthesised from glutamate in the body.

Due to its inhibitory function, GABA plays a vital plays role in anxiety. In this situation, when nerve signals fire too quickly and possess anxiety-inducing signals, it is activated and acts in order to slow the signals down and therefore reducing overwhelming feelings of anxiety. In individuals with anxiety disorders (e.g. post traumatic stress, panic disorder and generalised anxiety), it does not function as it should and this results in elevated levels of anxiety.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with 5-GABA.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum sensitivity detection limit of gamma aminobutyric acid (GABA) using current GABA ELISA kit was approximately 18.75 pg/ml. The dynamic range for this assay is 31.25 – 2,000 pg/ml.

ASSAY CHARACTERISTICS

– Analytical Specificity (Cross Reactivity): GABA (100%), ß-Alanine (0.50%), α-Aminobutyric acid (< 0.001%).
– Intra Assay Precision: 10.0 – 11.0%
– Inter Assay Precision: 14.0 – 16.0%
– Recovery: 104 – 111% (Male) and 105 – 118% (Female).

– Specificity: Highly specific for GABA, no cross reactivity or interference between GABA and analogues was detected.
– Recovery: Serum (97 – 104%), EDTA Plasma (95 – 104%), Heparin Plasma (95 – 100%).
– Linearity: Serum (87 – 99%), EDTA Plasma (86 – 100%), Heparin Plasma (88 – 104%).
– Precison Intra-Assay: CV < 4%.
– Precison Inter-Assay: CV < 4%.
– Stability: Less than 10%.

REFERENCES

1. Prefrontal cortical gamma-aminobutyric acid transmission and cognitive function: drawing links to schizophrenia from preclinical research. Biol Psychiatry. (2015) 77 (11): 929-39. Review. Tse M.T., et al.
2. Molecular aspects of age-related cognitive decline: the role of GABA-signaling. Trends Mol Med. (2015) 21 (7): 450-60. Review. McQuail J.A., et al.
3. Brain γ-aminobutyric acid: a neglected role in impulsivity. Eur J Neurosci. (2014) 39 (11): 1921-32. Review. Hayes D.J., et al.
4. Hypoxia and GABA shunt activation in the pathogenesis of Alzheimer’s disease. Neurochem Int. (2016) 92: 13-24. Review. Salminen A., et al.

ADDITIONAL INFORMATION

• Full Name: GABA ELISA Kit (Gamma-Aminobutyric Acid)
• Reactivity: Human
• Sample Type: Serum, Plasma, Biological Fluids, Tissue Homogenates
• Sensitivity: 18.75 pg/ml

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• Serotonin ELISA Kit
• Dopamine ELISA Kit
• 2-CAT (Adrenaline/Noradrenaline) ELISA Kit

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