Free 17 Beta Estradiol Saliva ELISA Kit


Human free 17 beta estradiol saliva ELISA kit is an accurate in vitro quantitative method for detecting free 17β estradiol (17 beta estradiol, 17b-diol, 17beta estradiol) in human saliva. This assay has a minimum sensitivity detection limit of 0.06 pg/ml.


17 beta estradiol which can also be referred to as 17beta estradiol, 17b-diol, 17β estradiol and even E2, is present in both males and females and is one of the most important natural estrogen in humans. In males estradiol is less well defined but it is thought to be vital in regulating the secretion of gonadotropin and fertility, whereas in females it is found to act as growth hormone for tissues which are related to the reproductive organs. The measurement of 17β estradiol has been used in helping to detect lack of estrogenes which are likely to be expressed in menopause, delayed puberty,  primary and secondary amenorrhoea. It can also be useful in evaluating fertility, detecting any menstrual irregularities and monitoring the ovarian follicular function during the induction phase of ovulation.

The following salivary 17beta estradiol ELISA kit has been useful in the diagnosis and treatment of a number of hormonal related sexual disorders and also in evaluating ovarian function, however, this test should not be used to assess the placental function during complicated pregnancy. In plasma the majority of estradiol is found to be bound to either sex hormone binding globulin (SHBG) or albumin, with only a small fraction 1-3% being present in the free form. It is this free estradiol portion which has the biologically active part during endocrine regulation, another important point is that it is this free hormone which is also released in equal amounts in saliva. Due to the fact that the levels of human 17 beta estradiol tend to fluctuate, this makes the use of repeatedly collected saliva samples in order to get an accurate profile of this hormone quite attractive. It also offers the added advantages of being non-invasive and relatively pain free to the patient when compared to extracting blood samples.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiterwells (96 Wells): Break apart strips that are coated with polyclonal anti-estradiol antibody.
  • Standard 0-5: Concentrations 0, 1, 5, 10, 50, 100 pg/ml, made up from human estradiol.
  • Control Low And Control High: Contains human 17 beta estradiol.
  • Enzyme Conjugate: Consisting of estradiol conjugated to horseradish peroxidase (HRP).
  • TMB Substrate Solution.
  • Stop Solution.
  • Wash Solution (Concentrated).


The minimum sensitivity detection limit of human free 17β estradiol (17b-diol, 17 beta estradiol) using current salivary 17beta estradiol ELISA kit was approximately 0.06 pg/ml. The dynamic range for this assay is 1.0 – 100.0 pg/ml.


– Salivary 17beta Estradiol (17 Beta Estradiol Saliva): ELISA
– Assay Dynamic Range: 0.6 – 100 pg/mL.
– Specificity (Cross Reactivity): Percentage indicates cross reactivity at 50% displacement compared to Estradiol. Estradiol-17ß (100%), Estrone (6.86%), Estriol (2.27%), Fulvestrant (0.9%), Testosterone (0.033%), 17-Hydroxyprogesterone (0.003%).
– Sensitivity: 0.6 pg/mL at the 95 % confidence limit.
– Intra Assay: 2.4 – 8.3%
– Inter Assay: 2.8 – 12.0%
– Recovery: 85.4 – 112.0%
– Linearity: 88.5 – 112.8%
– Drug Interferences: Patients being treated with the drug fulvestrant (Faslodex®) could lead to falsely elevated test results.
– Interfering Substances: Blood contamination greater than 0.16% that may be present in the saliva samples will affect results (this usually can be seen by the eye).
– High-Dose-Hook Effect: None


  1. Salivary estradiol as a surrogate marker for serum estradiol in assisted reproduction treatment. Clin Biochem. (2017) 50 (3): 145-149. Fiers T., et al.
  2. Progesterone and estradiol in the saliva and plasma during the menstrual cycle. Am J Obstet Gynecol. (1983) 147 (5): 557-62. Choe J.K., et al.
  3. Sensitive salivary estradiol assay for monitoring ovarian function. Clin Chem. (1990) 36 (10): 1769-73. Worthman C.M., et al.
  4. Saliva as a medium for investigating intra- and interindividual differences in sex hormone levels in premenopausal women. Cancer Epidemiol Biomarkers Prev. (2001) 10 (1): 59-64. Gann P.H., et al.


  • Full Name: Free 17 Beta Estradiol Saliva ELISA Kit
  • Reactivity: Human
  • Sample Type: Saliva
  • Sensitivity: 0.06 pg/ml



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