Full Name: FCoV ELISA Kit (Feline Corona Virus)
Sample Type: Serum, Plasma, Ascetic Fluid
Feline coronavirus (FCoV) is infectious to cats throughout the world and it is caused by a positive stranded RNA. Feline infectious peritonitis (FIP) exists in two different forms: FIP and FIPV. On the whole the majority of cats that are infected tend to recover fully and also develop immunity to corona virus. There is evidence that some cats can become carriers of the virus and therefore can infect other cats, however, there are a few cats which are unable to build immunity to corona virus and this can result in the disease developing into the fata form. FIP is a heterogeneous neoplastic disease, which means that it has different causes and often no definitive diagnosis. It predominantly affects the gastrointestinal tract with an extensive variable extent of lymphoid tissue involvement and morphologically, it is characterized by diffuse proliferation of spindle-shaped cells with pale eosinophilic cytoplasm (i.e., epithelial cells) in the lamina propria and submucosa that form a large hypercellular mass called a tumoroncosis. The disease can also involve the lung, liver, bone marrow and central blood pressure , and can lead to other cancer types in these organs. It is a cancer, which commonly presents with no symptoms in the early stages; however, it can cause painful bowel cramps, fatigue, fever and weight loss.
This fatal form of FIP is a progressive, chronic disease which can be characterised by; loss of appetite, antibiotic-resistant fever, lethargy, anaemia and weakness. In the majority of cases, the linings of the chest and stomach cavities are inflamed. Only a small number (approx. 5%) of the cats which are infected with the coronavirus actually develop the infectious peritonitis.
FCoV ELISA kit is intended for detecting antibodies against feline corona virus (FCoV)(mostly glycoproteins) in plasma, serum and ascetic fluid.
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Strips. Coated with FCoV proteins.
- Strip Holder.
- ELISA Buffer.
- HRPO Conjugated Anti-Species Antibodies.
- Positive Control (Freeze Dried).
- Negative control (Freeze Dried).
- Wash Solution (200x Concentrated).
- Substrate A.
- Substrate B.
- Stop Solution.
- Plastic Cover Seal.
The standard range of antibodies to feline corona virus (FCoV) using current FCoV ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.
– Positive control should give an extinction > 0.800 OD units and an end point titer > 270.
– Negative control should give an OD < 0.400 and end point titer < 30.
– End point titer of the sample is the dilution that gives an extinction just above 0.350 OD units at 450 nm.
- Utility of feline coronavirus antibody tests. J Feline Med Surg. (2015) 17 (2):152-62. Addie D.D., et al.
- Comparison of serologic techniques for the detection of antibodies against feline coronaviruses. J Vet Diagn Invest. (2008) 20 (1): 45-50. Pratelli A.
- Relevance of feline interferon omega for clinical improvement and reduction of concurrent viral excretion in retrovirus infected cats from a rescue shelter. Res Vet Sci. (2013) 94 (3): 753-63. Gil S., et al.
- Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests. J Virol Methods. (2014) 196: 1-6. Takano T., et al.
OTHER RELATED ELISA KITS
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- FPV (Feline Panleukopenia Virus) Ab ELISA Kit (Feline)
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