FCV ELISA Kit (Feline Calici Virus)

Full Name: FCV ELISA Kit (Feline Calici Virus)
Reactivity: Feline
Sample Type: Serum, Plasma
Sensitivity: Qualitative

BACKGROUND

Cat flu is also known as feline calicivirus (FCV) infection, is a main cause of upper respiratory diseases in cats. It can harm to the respiratory, nasal and oral areas as well as tongue ulcers, digestive system and the skeletal muscles. It is a common virus found globally and is responsible for causing the feline infectious peritonitis (FIP) disease in cats. FIP is a contagious and fatal disease of cats caused by calicivirus. Feline calicivirus is also known as feline herpes virus. It is not known what triggers the development of FIP since the virus has been found in healthy cats that never develop the disease. It can be contracted via close contact with an infected cat or contaminated objects that were used to care for an infected cat like bedding, toys, food bowls etc.

At present there have been at least 40 different strains of FCV that have been discovered and the severity or the virulence of the disease resulting from the different strains can vary considerably from mild to severe. It is typically transmitted between animals through direct contact, or via airborne transmission through an infected animal’s respiratory secretions or saliva. Transmission of FCV can also occur via urine-contaminated surfaces or contaminated drinking water if the tap water contains viable virus.

INTENDED USE

FCV ELISA kit can be used for measuring antibodies against feline calici virus (FCV or feline calicivirus) in samples of serum or plasma.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Strips. Coated with FCV proteins.
Positive Control (Freeze Dried).
Negative control (Freeze Dried).
Strip Holder.
ELISA Buffer.
HRPO Conjugated Anti-Species Antibodies.
Wash Solution (200x Concentrated).
Substrate A.
Substrate B.
Plastic Cover Seal.
Stop Solution.

SENSITIVITY

The standard range of antibodies to feline calicivirus (FCV) using current FCV ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Weak positive control should give an extinction ≥ 1.500 OD and an endpoint titer ≥ 90.
– Negative control should give an OD ≤ 0.600 and an endpoint titer =< 30.
– A titer ≥ 270 is considered to be protective, under normal virus pressure.
– Summary:
(i). < 30 = no antibodies found.
(ii). > 90 = antibodies found, retest in 3 months. (probably shedding FCV)
(iii). > 270 = high titer, considered to be protective.

REFERENCES

Inactivation of caliciviruses. Appl Environ Microbiol. (2004) 70 (8): 4538-43. Duizer E., et al.
Feline inflammatory polyps: historical, clinical, and PCR findings for feline calici virus and feline herpes virus-1 in 28 cases. J Feline Med Surg. (2002) 4 (4): 195-9. Veir J.K., et al.
Treatment of cats with ocular disease attributable to herpesvirus infection: 17 cases (1983-1993). J Am Vet Med Assoc. (1995) 207 (5): 599-603. Stiles J.
Chronic oral infections of cats and their relationship to persistent oral carriage of feline calici-, immunodeficiency, or leukemia viruses. Vet Immunol Immunopathol. (1991) 29 (1-2): 1-14. Tenorio A.P., et al.

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