FPV ELISA Kit (Feline Panleukopenia Virus)

Full Name: FPV ELISA Kit (Feline Panleukopenia Virus)
Reactivity: Feline
Sample Type: Serum, Plasma
Sensitivity: Qualitative

BACKGROUND

Feline panleukopenia virus (FPV), is a life threatening and highly contagious viral disease found in cats. It is primarily responsible for affecting the rapid dividing blood cells which can be found in the intestinal tract, bone marrow and stem cells (developing fetus). FPV can be passed from cat to cat through close contact with contaminated surfaces or feces or through contact with an infected animal’s blood, urine, saliva and other secretions. Cats with FIP typically show signs of pain and a reluctance to move. Other common symptoms include discharge from the eyes, nose, mouth or genitals that may spread to the paws and tail. In some cases, cats can lose their appetite and start vomiting. Treatment usually consists of antibiotics but sometimes blood transfusions are necessary to offset low white blood cell counts (a hallmark sign). The prognosis for cats is good .This condition is fairly common in young cats with a history of being at risk for exposure to ticks.

For diagnosis of FPV infection and vaccination control, measuring antibody titre is one of the most common procedure used. This involves having a virus being attached to the solid phase via monoclonal antibodies that are able to catch the antibodies which have been induced as a result of the either infection or vaccination. Antibody titers which are greater than 1:1350 dilutions are then considered to be protected. Other test are available to analyse the virus in faeces, but the isolation step can be quite time consuming and the test itself is expensive. Also, any recent vaccinations against panleukopenia will result in producing a false positive.

INTENDED USE

FPV ELISA kit is intended for analysing antibodies against feline panleukopenia virus (FPV) in samples of serum or plasma.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Strips: Coated with monoclonal FPV antibodies.
Strip Holder.
Inactivated FPV.
ELISA Buffer.
HRPO Conjugate Anti-Species Antibodies
Positive Control.
Negative Control.
Substrate A.
Substrate B.
Wash Solution (200x Concentrated).
Stop Solution.
Plastic Cover Seal.

SENSITIVITY

The standard range of feline panleukopenia virus (FPV) using current FPV ELISA Kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Positive control should give an OD (450nm) > 1,000.
– Negative control must give an OD (450nm) lower than 0,300.
– A sample is considered positive when the measured extinction is 0.150 higher than the OD of the negative control.

REFERENCES

Detection of protective antibody titers against feline panleukopenia virus, feline herpesvirus-1, and feline calicivirus in shelter cats using a point-of-care ELISA. J Feline Med Surg. (2011) 13 (12): 912-8. Digangi B.A., et al.
Evaluation of an in-house dot enzyme-linked immunosorbent assay to detect antibodies against feline panleukopenia virus. J Feline Med Surg. (2014) 16 (10): 805-11. Mende K., et al.
Feline parvovirus infection and associated diseases. Vet J. (2014) 201 (2): 150-5. Review. Stuetzer B. and Hartmann K.
Detection of feline panleukopenia virus using a commercial ELISA for canine parvovirus. Vet Ther. (2009) 10 (4): E1-6. Abd-Eldaim M., et al.
Vaccination against Feline Panleukopenia: implications from a field study in kittens. BMC Vet Res. (2012) 8: 62. Jakel V., et al.

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