Diphtheria Toxoid IgG ELISA Kit

Full Name: Diphtheria Toxoid IgG ELISA Kit
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 0.004 IU/ml


Diphtheria toxoid bacteria has the capability to generate a potent toxin which leads to irritation of the throat, nose and skin for individuals who come in contact with it. Left untreated, the exotoxin can be deadly as it causes intense tissue and organ damage due to the severe inflammation.

There are vaccines to protect us against diphtheria, with these vaccines and proper preventive measures, this deadly disease can be stopped from spreading.


Human diphtheria toxin IgG ELISA kit is designed for detecting in-vitro levels of human IgG antibodies against diphtheria toxin (diphtheria toxoid IgG) using samples of plasma and serum. This assay has a minimum analytical sensitivity limit of 0.004 IU/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Diphtheria Toxoid antigen coated microtiter strips.
  • Calibrators A-D.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Stop Solution.
  • Sample Diluent.
  • Washing Buffer (10x).


The minimum detection sensitivity level of human IgG antibodies to diphtheria toxin (diphtheria toxoid IgG) using current diphtheria toxin IgG ELISA kit was 0.004 IU/ml. The dynamic range for this assay is 0.01 – 1.0 IU/ml.


– Intra-Assay-Precision: 7.5 %
– Inter-Assay-Precision: 4.9 %
– Inter-Lot-Precision: 2.3 – 7.4 %
– Analytical Sensitivity: 0.004 IU/mL
– Recovery: 96 – 102 %
– Linearity: 78 – 133 %
– Cross-Reactivity: No cross-reactivity to Clostridium tetani.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL.
– Clinical Specificity: 94 %
– Clinical Sensitivity: 94 %
– Measuring Range: 0.01 – 1.0 IU/mL


  1. Evaluation and characterisation of A and B fragments of Corynebacterium diphtheriae toxin towards recombinant diphtheria vaccine. Indian J Med Microbiol. (2013) 31 (1): 3-9. Abulmagd S., et al.
  2. Comparison of seven commercial enzyme-linked immunosorbent assays for the detection of anti-diphtheria toxin antibodies. Eur J Clin Microbiol Infect Dis. (2013) 32 (7): 891-7. Zasada A.A., et al.
  3. Preparation of a diphtheria toxin-pullulan conjugate that elicits good IgG antibody production with poor IgE synthesis. Vaccine. (1990) 8 (1): 65-9. Yamaya S., et al.
  4. Some properties of IgG against diphtheria toxin synthesized in Xenopus oocytes containing mRNA from hybridoma. Biochim Biophys Acta. (1985) 825 (2): 188-98. Hara Y., et al.
  5. Reducing anti-DT IgG concentrations to improve the efficacy of a diphtheria_fusion protein. Anticancer Drugs. (2008) 19 (10): 1007-11. Hall P.D., et al.


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