Full Name: Chlamydia Pneumoniae IgM ELISA Kit
Sample Type: Plasma, Serum
Sensitivity: diagn. 83.3%
Chlamydia pneumoniae has a cell wall containing LPS and outer membrane proteins that facilitate adhesion and entry into respiratory epithelial cells. Within cells, C. pneumoniae inhibits phagolysosome fusion and evades immune detection. It can also enter a persistent, non-replicative state. The type III secretion system injects effector proteins that manipulate host cell processes to promote infection.
Like other Chlamydia species, it has a biphasic developmental cycle consisting of elementary bodies and reticulate bodies. The elementary body is the infectious form, small and spore-like with a rigid cell wall. Elementary bodies attach to and enter host cells, then transition into larger reticulate bodies that proliferate by binary fission within an inclusion vacuole. Reticulate bodies re-differentiate into elementary bodies that are released to infect new cells.
Acute C. pneumoniae respiratory infections are generally mild and self-limiting, causing pharyngitis, sinusitis, or bronchitis. Seroprevalence studies indicate most people are infected at some point. Reinfection is common since natural immunity is short-lived.
Unusual for an obligate intracellular bacterium, C. pneumoniae can spread directly between hosts via respiratory droplets. But it can also disseminate systemically and has been detected in arteries, causing inflammation implicated in atherosclerosis. The tendency for persistent infection and inflammation likely underlies C. pneumoniae’s association with chronic illnesses.
Human Chlamydia pneumoniae IgM ELISA kit is a protocol designed for in-vitro measurement of human IgM class antibodies against Chlamydia pneumoniae (Chlamydia pneumoniae-IgM) present in plasma or serum. This assay has a minimum analytical sensitivity limit to a diagn. 83.3%.
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Chlamydia pneumoniae Coated Microplate (IgM).
- Positive Control.
- Cut-off Control.
- Negative Control.
- IgM Sample Diluent.
- Chlamydia pneumoniae anti-IgM Conjugate.
- TMB Substrate Solution.
- Stop Solution.
- Washing Buffer (20x Conc.).
The minimum detection sensitivity level of human IgM class antibodies to Chlamydia pneumoniae using current Chlamydia pneumoniae IgM ELISA kit was to a diagn. 83.3%. The dynamic range for this assay is to a specific cut-off point.
– Equivocal: 9 – 11 U
– Positive: > 11 U
– Cut-Off: 10 U
– Negative: < 9 U
– Diagnostic Specificity: 99.26%
– Diagnostic Sensitivity: 90.00%
– Cross Reactivity: No false-positive observed.
– Interferences: No interferences identified using with hemolytic, lipemic or icteric samples.
- The validity of the criteria for primary infection of Chlamydophila-pneumoniae in children by measuring ELISA IgM antibodies. J Infect Chemother. (2012) 18 (3): 308-12. Kamata A., et al.
- Strong correlation in the serum levels of IgM rheumatoid factor and IgM anti-Chlamydia pneumoniae antibody. Lupus. (2009) 18 (12): 1124. Iwamoto M., et al.
- Detection of IgM antibodies to Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydiapsittaci from Japanese infants and children with pneumonia. In Vivo. (1992) 6 (6): 601-4. Numazaki K., et al.
- High seroprevalence of Mycoplasma pneumoniae IgM in acute Q fever by enzyme-linked immunosorbent assay (ELISA). PLoS One. (2013) 8 (10): e77640. Lai CH, et al.
- Assay of Chlamydia pneumoniae-specific IgM antibodies by ELISA method–reduction of non-specific reaction and resetting of serological criteria by measuring IgM antibodies–. Jpn J Infect Dis. (2009) 62 (4): 260-4. Kishimoto T., et al.
OTHER RELATED ELISA KITS
- Chlamydia Pneumoniae-IgA ELISA Kit
- Chlamydia Pneumoniae-IgG ELISA Kit
- Chlamydia Trachomatis-IgM ELISA Kit
Online Enquiry Form
"*" indicates required fields