Chlamydia Pneumoniae IgM ELISA Kit


Human Chlamydia pneumoniae IgM ELISA kit is a protocol designed for in-vitro quantitative measurement of human IgM class antibodies against Chlamydia pneumoniae (Chlamydia pneumoniae-IgM) present in plasma or serum. This assay has a minimum analytical sensitivity limit to a diagn. 83.3%.


Chlamydia pneumoniae has been implicated a large number of chronic illness for example asthma, multiple sclerosis, arthritis, atherosclerosis and many others. However, it has been found to be a common cause of both lower and upper respiratory illnesses such as pneumonia, pharyngitis, sinusitis and bronchitis in both adults and children. The diagnosis of acute infections is quite well established in most laboratories, however the diagnosis of the chronic infections pose many difficulties since the infections can be caused by a multitude of factors. Typically, diagnosis is usually based on either direct microscopy or serology (such as ELISA) methods. Atypical pneumonia, sinusitis and bronchitis are the most frequent respiratory diseases which are caused by C. pneumonia, persistent respiratory infections such as these are accepted as good indicators for any possible Chlamydia infections.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Chlamydia pneumoniae Coated Microplate (IgM).
  • Positive Control.
  • Cut-off Control.
  • Negative Control.
  • IgM Sample Diluent.
  • Chlamydia pneumoniae anti-IgM Conjugate.
  • TMB Substrate Solution.
  • Stop Solution.
  • Washing Buffer (20x Conc.).


The minimum detection sensitivity level of IgM class antibodies against Chlamydia pneumoniae using current human Chlamydia pneumoniae-IgM ELISA kit was to a diagn. 83.3%. The dynamic range for this assay is to a specific cut-off point.


– Chlamydia Pneumoniae ELISA: IgM
– Equivocal: 9 – 11 U
– Positive: > 11 U
– Cut-Off: 10 U
– Negative: < 9 U
– Diagnostic Specificity: 99.26%
– Diagnostic Sensitivity: 90.00%
– Cross Reactivity: No false-positive observed.
– Interferences: No interferences identified using with hemolytic, lipemic or icteric samples.


  1. The validity of the criteria for primary infection of Chlamydophila pneumoniae in children by measuring ELISA IgM antibodies. J Infect Chemother. (2012) 18 (3): 308-12. Kamata A., et al.
  2. Strong correlation in the serum levels of IgM rheumatoid factor and IgM anti-Chlamydia pneumoniae antibody. Lupus. (2009) 18 (12): 1124. Iwamoto M., et al.
  3. Detection of IgM antibodies to Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydiapsittaci from Japanese infants and children with pneumonia. In Vivo. (1992) 6 (6): 601-4. Numazaki K., et al.
  4. High seroprevalence of Mycoplasma pneumoniae IgM in acute Q fever by enzyme-linked immunosorbent assay (ELISA). PLoS One. (2013) 8 (10): e77640. Lai CH, et al.
  5. Assay of Chlamydia pneumoniae-specific IgM antibodies by ELISA method–reduction of non-specific reaction and resetting of serological criteria by measuring IgM antibodies–. Jpn J Infect Dis. (2009) 62 (4): 260-4. Kishimoto T., et al.


  • Full Name: Chlamydia Pneumoniae IgM ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: diagn. 83.3%



Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford