D-Dimer ELISA Kit (D2D)
INTENDED USE
Human D-Dimer ELISA kit can measure concentrations of D2D (D-Dimer) present is plasma, serum, biological fluids or tissue homogenate samples.
INTRODUCTION
D dimer often referred to as D2D or D D is a substance produced when fibrin, a component of blood clots breaks down. Sensitive tests using D dimer can help rule out thrombosis and disseminated coagulation (DIC).
D dimer consists of two interconnected D fragments that are formed from the breakdown of fibrin during the process of a clot. When the body activates the fibrinolytic system to dissolve a thrombus D dimer is generated. This molecule contains two units of monomers. The presence in the blood indicates both clot formation and ongoing activation of coagulation.
Measuring levels of D dimer is valuable in excluding thromboembolism and pulmonary embolism in low-risk populations. If levels are within the range, it highly suggests that there is no presence of a clot. Additionally D dimer tests are useful in diagnosing DIC – a condition characterized by clotting and excessive fibrinolysis. Elevated levels correspond with the severity of DIC. It can aid in monitoring this conditions progression. However, it’s important to note that while elevated levels indicate issues mild increases may also be caused by other conditions. Therefore, careful interpretation, within a context is necessary when evaluating results.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti-D2D antibody.
- Standards: Lyophilized recombinant.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
TYPICAL RESULTS
For this D-Dimer ELISA kit, it is recommended that a standard curve is generated for each assay carried out.
Standard Curve: 0, 78.125, 156.25, 312.5, 625, 1250, 2500, 5000 pg/ml.
Reactivity: Human
Sensitivity: 46.875 pg/ml
Range: 78.125 – 5000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.
ASSAY CHARACTERISTICS
– Specificity: Highly specific for D2D, no cross reactivity or interference between D2D and analogues was detected.
– Recovery: Serum (90 – 105%), EDTA Plasma (82 – 93%), Heparin Plasma (80 – 99%).
– Linearity: Serum (88 – 105%), EDTA Plasma (82 – 93%), Heparin Plasma (80 – 99%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%
REFERENCES
- D-dimer: An Overview of Hemostasis and Fibrinolysis, Assays, and Clinical Applications. Adv Clin Chem. (2015) 69: 1-46. Olson J.D.
- D-Dimer Testing: Laboratory Aspects and Current Issues. Methods Mol Biol. (2017) 1646: 91-104. Thachil J., et al.
- D-dimer assays in diagnosis and management of thrombotic and bleeding disorders. Semin Thromb Hemost. (2012) 38 (7): 673-82. Bates S.M.
- D-dimer: standardization versus harmonization. Thromb Haemost. (2006) 95 (3): 399-400. Dempfle C.E.
ADDITIONAL INFORMATION
- Full Name: D-Dimer ELISA Kit (D2D)
- Reactivity: Human
- Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
- Sensitivity: 46.875 pg/ml
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