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Chemerin ELISA Kit (CHEM)

Full Name: Chemerin ELISA Kit (CHEM)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 0.094 ng/ml

INTRODUCTION

Chemerin is an adipokine and chemokine implicated in diverse physiological processes ranging from immune cell recruitment to metabolism and adipogenesis. While expressed in various tissues, active chemerin is largely produced from precursor prochemerin by inflammatory and coagulation serine proteases. This links its regulated activation to tissue injury and infection. The chemerin receptor CMKLR1 expresses on numerous immune cell types, conferring pleiotropic actions that make it an integral node connecting inflammation to metabolic homeostasis.

As a chemoattractant, chemerin plays key roles in tissue leukocyte recruitment after inflammation or damage. By stimulating chemotaxis via CMKLR1, it directs efficient immune cell migration to lymphoid tissues and sites of injury.  Furthermore, its effects on macrophage polarity and secretion of proresolving lipid mediators implicate immunomodulatory roles. Hence, chemerin acts as early checkpoint shaping key aspects of inflammation.

However, chemerin signaling also holds significant metabolic consequences in obesity which constitutes a chronic inflammatory state. Its production in adipocytes regulated by cytokines and factors like norepinephrine and insulin connects nutrient stresses to adipose dysfunction. By impairing insulin signaling and glucose uptake while enabling adipogenesis and fat accumulation, chronically elevated chemerin contributes to obesity-induced inflammation, insulin resistance, and associated complications. Hence appropriate regulation is vital for health.

INTENDED USE

Human chemerin ELISA kit can measure concentrations of CHEM (chemerin, TIG-2, TIG2, retinoic acid receptor, tazarotene induced 2) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-chemerin antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

For this chemerin ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.
Reactivity: Human
Sensitivity: 0.094 ng/ml
Range: 0.125 – 10 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for CHEM, no cross reactivity or interference between CHEM and analogues was detected.
– Recovery: Serum (87 – 102%), EDTA Plasma (85 – 105%), Heparin Plasma (86 – 103%).
– Linearity: Serum (85 – 98%), EDTA Plasma (87 – 99%), Heparin Plasma (82 – 100%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

  1. Chemerin/chemR23 association with endothelial-mesenchymal transition in diabetic nephropathy. Int J Clin Exp Pathol. (2017) 10 (7): 7408-7416. Wang X., et al.
  2. The antimicrobial activity of chemerin-derived peptide p4 requires oxidative conditions. J Biol Chem. (2019) 294 (4): 1267-1278. Godlewska U., et al.#
  3. Chemerin, a novel adipokine that regulates adipogenesis and adipocyte metabolism. J Biol Chem. (2007) 282 (38): 28175-88. Goralski K.B., et al.
  4. Tandem Repeat of a Short Human Chemerin-Derived Peptide and Its Nontoxic d-Lysine-Containing Enantiomer Display Broad-Spectrum Antimicrobial and Antitubercular Activities. J Med Chem. (2021) 64 (20): 15349-15366. Pratap Verma D., et al.

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